摘要
目的:建立噁唑酮诱导的大鼠结肠炎模型,观察阿泰宁对噁唑酮诱导的大鼠结肠炎的治疗作用及机制.方法:经直肠注入噁唑酮建立大鼠溃疡性结肠炎模型.将大鼠随机分为:空白对照组(正常组,n=8),模型组(n=10),美沙拉秦组(正常组,n=10),阿泰宁组(n=10),治疗21d后处死动物,肉眼观察结肠病变,分别测体质量、结肠湿质量、脾脏质量和结肠组织病理学变化,ELISA法测定大鼠血清IL-1β、IL-10、TNF-α以及结肠黏液内容物sIgA含量,考马斯亮兰法测定血清总蛋白,溴甲酚绿比色法测定白蛋白以及肠道菌群培养.结果:模型组、阿泰宁组和美沙拉秦组大鼠体质量均比正常组显著降低(均P<0.01).美沙拉秦组和阿泰宁组血清球蛋白含量、结肠湿质量指数较模型组差异显著(29.9±5.7,29.1±5.4vs23.7±9.5;6.0±0.9,6.2±0.4vs7.4±1.6,均P<0.05).模型组大鼠血清IL-1β和TNF-α含量较正常组、美沙拉秦组和阿泰宁组差异显著(44.6±17.2vs8.8±7.9,14.5±4.7,8.6±3.4,均P<0.01;33.5±7.2vs22.6±6.7,22.3±9.2,24.4±10.8,均P<0.05).模型组大鼠血清IL-10含量较正常组、阿泰宁组差异显著(101.5±35.8vs280.5±36.1,271.3±33.8,P<0.01).阿泰宁组脾脏指数、sIgA含量比正常组显著升高(3.4±0.8vs2.7±0.3;46.0±20.3vs23.4±18.5,均P<0.05),而美沙拉秦组差异不显著.较模型组双歧杆菌数量,阿泰宁治疗后明显上升,梭杆菌数量明显下降(9.7±0.1vs9.3±0.2;3.7±0.3vs5.8±0.7,均P<0.01).结论:阿泰宁能够有效治疗噁唑酮诱导的大鼠结肠炎.
AIM： To establish a model of ulcerative colitis induced by oxazolone in rats, to observe therapeutic effects of A Tai Ning for treatment of oxazolone-induced ulcerative colitis and to investigate its mechanism.METHODS： Intrarectal injection of oxazolone was performed to induce ulcerative colitis in rats. Rats were randomly assigned to control group （n = 8）, model group （n = 10）, mesalamine group and A Tai Ning group （n = 10）. After 21 days’s treatsment, all the animals were sacri-ficed and their body weight, weight of fresh ulcerative tissues, and the weight of spleen were measured. Histopathological changes in ulcer-ative tissues were determined using ELISA to specify the amount of IL-1β, IL-10 and TNF-α in rat serum, and sIgA in the contents of ulcerative tissues. The content of total protein and albumin in serum were determined using the Coomassie brilliant Blue and the Bromocresol Green colori-metric method, respectively. The intestinal bac-terial flora was determined using bromocresol green method. RESULTS： Compared with the control group, all test groups including model group, A Tai Ning and mesalamine groups showed signifi-cant weight loss （all P 〈 0.01）. Compared with the control group, there were significant differ-ences in colonic wet weight index and the quan-tity of serum globulin both in A Tai Ning and Mesalamine groups （29.9 ± 5.7, 29.1 ± 5.4 vs 23.7 ± 9.5; 6.0 ± 0.9, 6.2 ± 0.4 vs 7.4 ± 1.6, all P 〈 0.05）. Serum IL-1β and TNF-α levels in the model group were significantly different from those in-the control group, the Mesalamine group or the A Tai Ning group （44.6 ± 17.2 vs 8.8 ± 7.9, 14.5 ± 4.7, 8.6 ± 3.4, both P 〈 0.01; 33.5 ± 7.2 vs 22.6 ± 6.7, 22.3 ± 9.2, 24.4 ± 10.8, both P 〈 0.05）. The serum IL-10 level in the model group was significantly lower compared with the control group or A Tai Ning group （101.5 ± 35.8 vs 280.5 ± 36.1, P 〈 0.01）, whereas the A Tai Ning group showed higher levels of IL-10 compared with the control group （271.3 ± 33.8 vs 101.5 ± 35.8, P 〈 0.01）. The spleen mass index and the content of sIgA in rat ulcers in the A Tai Ning group were significantly in-creased compared with the control group （3.4 ± 0.8 vs 2.7 ± 0.3; 46.0 ± 20.3 vs 23.4 ± 18.5, both P 〈 0.05）, but no significant difference was detected between the Mesalamine group and A Tai Ning group. Compared with the model group, the level of Bifidobacterium in the A Tai Ning groupwas remarkedly increased while the level of Fu-sobacterium was decreased greatly （9.7 ± 0.1 vs 9.3 ± 0.2; 3.7 ± 0.3 vs 5.8 ± 0.7, both P 〈 0.01）. CONCLUSION： A Tai Ning is effective for oxa-zolone induced-ulcerative colitis in rats.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第27期3036-3042,共7页
World Chinese Journal of Digestology
基金
系列微生态生物新药高技术产业化示范工程资助项目
No.发改高技[2004]2078~~
关键词
阿泰宁
酪酸梭菌
噁唑酮
溃疡性结肠炎
A Tai Ning
Clostridium butyricum
Oxa-zolone
ulcerative colitis
