摘要
以健康脐带血为来源，采用３％明胶沉降法分离获取ＬＡＫ前体细胞，并在培养体系中增加植物血凝素诱导因子．结果：该法与常规淋巴细胞分层液分离法均能从脐带血中获取大量的ＬＡＫ前体细胞，且两者体外增殖情况及杀瘤活性并无明显差异（Ｐ＞０．０５）．提示新法简单快速，能从脐带血中制备出大量高抗肿瘤活性的ＬＡＫ细胞．
With healthy umbilical blood as a source, pre LAK cells were acquired by using a method of 3% gelatin precipitation and added in the culture system were some inducing factors like phytohematoagglutinin (PHA). Result: a large number of pre LAK cells can be acquired from the umbilical blood by both gelatin precipitation and regular Ficoll Hypaque centrifugation methods, between which there was no marked difference (P>0.05) for external proliferation and killing activities. All this suggested that the new method, simple and speedy, can prepare a huge number of anti tumor activity LAK cells.
出处
《昆明医学院学报》
1997年第3期74-76,共3页
Journal of Kunming Medical College
关键词
LAK细胞
胎血
植物血凝素
肿瘤
免疫疗法
LAK cells, Fetal blood, Phytohemotoagglutinin (PHA), Gelatin, Tumor
