摘要
目的:研究尿中修饰核苷检测在肝细胞性肝癌(HCC)诊断中的价值。方法:应用高效毛细管电泳法(HPCE)分别检测30名正常人、21例未发生转移的肝细胞性肝癌患者手术前后、21例肝硬化患者、10例肝良性肿瘤患者尿中5-甲基尿苷(M5U)、次黄嘌呤核苷(I)、N4-乙酰胞苷(Ac4c)、黄嘌呤核苷(X)四种修饰核苷的水平。结果:肝细胞性肝癌患者术前尿中M5U、I、Ac4c、X四种核苷的平均水平明显高于术后(P<0.01)。肝细胞性肝癌患者尿中四种核苷的平均水平明显较良性肿瘤患者、肝硬化患者、正常人高(P<0.05或P<0.01)。良性肿瘤患者、肝硬化患者、正常人尿中四种核苷的水平差异无显著性(P>0.05)。肝细胞性肝癌患者尿中四种核苷的平均水平同AFP无明显相关性(P>0.05),同肿瘤体积无明显相关性(P>0.05)。结论:尿中修饰核苷检测对肝细胞性肝癌的诊断有一定意义,四种修饰核苷含量对鉴别肝脏良恶性肿瘤也有一定临床意义。
Objective： To investigate the value for detecting the modified nucleosides diagnosing the heaptocellular carcinoma. Methods： Four kinds of urinary nucleosides （MSU in urine for I, Ac4c, X） from 21 preoperative and postoperative patients with primary heaptocellular carcinoma were detected. Ten cases with benign tumor of liver, 21 cases with cirrhosis of liver and 30 healthy persons were examined with high performance capillary electrophoresis（HPCE）. Results： The average level of 4 kinds of nucleosides from preoperative patients with heaptocellular carcinoma was higher than that from postoperative patients with heaptocellular carcinoma （P〈0.01）. The average level of 4 kinds of nucleosides from patients with heaptocellular carcinoma was higher than that from patients with benign tumor of liver, patients with cirrhosis of liver and 30 healthy persons （P〈0.05 or 0.01）. The average level from patients with benign tumor of liver, patients with cirrhosis of liver and 30 healthy persons had no significant difference （P〉0.05）. There was no significant correlation between the average level of 4 kinds of nucleosides and AFP （P〉0.05） or the tumor size （P〉0.05）. Conclusion： Urinary modified nucleosides may be helpful in diagnosing the heaptocellular carcinoma. The average level of 4 kinds of nucleosides has clinical significance in differentiating benign and malignant hepatic tumors.
出处
《温州医学院学报》
CAS
2008年第5期456-458,共3页
Journal of Wenzhou Medical College
关键词
核苷类
尿
癌
肝细胞
电泳
毛细管
nucleosides, urine
carcinoma, hepatocellular
electrophoresis, capillary
