摘要
利用SSR标记分析了贵州常用玉米自交系的遗传多样性。从50对引物中筛选到10对核心引物,10对引物在35个供试材料中共检测出75个等位基因位点,每对引物检测出5~14个,平均7.5个,多态性信息量为0.739。35个自交系之间的遗传相异系数变化范围为0.154~0.838,平均为0.513。聚类结果表明,35个自交系划分为7个类群,分类结果与系谱来源基本一致。贵州地方玉米种质被划分到不同类群中去,表明地方玉米种质具有广泛的遗传基础。
Genetic variation was analyzed of 35 maize inbred lines in Guizhou with SSR. Selected 10 coreprimers which could test 75 allele sites in sample of 35 inbred lines. The average number of allele per SSR locus was 7.5 with arrange from 5 to 14. The average of polymorphic information content (PIC) was 0.739. Gene locus among the 35 inbred lines ranged from 0.154 to 0.838 with an average of 0.513. The clustering results'showed that the inbred lines could be classified into 7 groups. The clustering results were consistent with the groups based on the available. The local germplasm lines could be divided into three heterotic groups, showed the local germplasm had wide genetic basis.
出处
《玉米科学》
CAS
CSCD
北大核心
2008年第5期11-15,共5页
Journal of Maize Sciences
基金
贵州省自然科学基金项目(2004-3026)