摘要
研究氯霉素、氨苄青霉素、卡那霉素三种抗生素和D-木糖、D-甘露糖两种糖类对乳酸杆菌生长的抑制效果,探讨相应标记基因作为乳酸杆菌RL01基因工程筛选标记的可行性。结果表明:(1)低浓度(5mg/L)的氯霉素和氨苄青霉素能显著抑制和杀灭乳酸杆菌,卡那霉素在5mg/L-400mg/L浓度范围内不能抑制乳酸杆菌的生长。氯霉素抗性基因和氨苄青霉素抗性基因(Ampr)适合作为菌株RL01的筛选标记基因,卡那霉素抗性基因(Kanr)不适合。(2)乳酸杆菌RL01能够利用D-甘露糖作为碳源,磷酸甘露糖异构酶基因(pmi)不适合作为乳酸杆菌的筛选标记。D-木糖作为碳源时,乳酸杆菌的生长受到明显抑制,木糖异构酶基因(xylA)有可能作为该菌株的食品级筛选标记。
In order to find the possibility for the corresponding marker genes to be used on the genetic manipulation of a Lactobacillus lactis, the inhibition effects on the growth of the Lactobacillus lactis strain RL01 were studied by three antibiotics including chloramphenicol, ampicillin and kanamycin and two carbohydrates such as Dmannose and D-xylose. Results showed： （1） Chloramphenicol and ampicillin with low concentration （5 mg/L） could inhibit and kill the Lactobacillus lacti notably, but kanamycin with concentrations among 5 mg/L-400 mg/L could not inhibit the growth of the strain. Thus the chloramphenicol resistant gene and ampicillin resistant gene （Amp^r） are suitable to use as the selected marker genes for Lactobacillus lacti LR01 while kanamycin resistant gene （Kan^r） not. （2） The Lactobacillus lacti LR01 could use D-mannose as carbon source and the phosphomannose isomerase gene （pint） is not suitable to use as its selected marker. When D-xylose as carbon source, the growth of LR01 strain were inhibited notably. The xylose isomerase （xylA） may be used as the food-grade selected marker gene for this strain.
出处
《食品研究与开发》
CAS
北大核心
2008年第10期35-38,共4页
Food Research and Development
基金
浙江省自然科学基金(Y304346)
浙江省食品科学与工程重中之重重点学科开放课题
关键词
乳酸杆菌
基因工程
筛选标记
Lactobacillus lactis
genetic manipulation
selected markers
