摘要
在甘肃省临泽县盐渍地采集紫花苜蓿耐盐根瘤菌，编号为GL007，它能在pH值为14，NaCl浓度为1．539mol／L，Na2SO4浓度为1．971mol／L的YMA培养基上正常生长。经温室蛭石盆栽试验对5个紫花苜蓿品种进行接种效果的研究。结果表明，该根瘤菌与5个紫花苜蓿品种都能共生结瘤，表现出共生效应的多样性。接种该耐盐根瘤菌的紫花苜蓿与不接种的对照相比其结瘤数增加了11．4％～77．7％，有效瘤重增加了26．4％～80．8％，地上生物量鲜重增加了45．8％～87．5％，地上生物量干重增加了49．1％～106．3％，粗蛋白质含量增加了1．5％～13．5％，初步筛选出的与耐盐根瘤菌GL007最佳匹配的紫花苜蓿品种是金黄后，其地上生物量鲜重、干重、粗蛋白质含量分别比对照增加了82．6％，106．3％和13．5％，与对照有显著差异。
We collected saline tolerance rhizobia from saline soil Medicago sativa in Linze county of Gansu province which can growth well in the culture medium （pH 14, NaCl 1. 539 mol/L and Na2SO4 1. 971 mol/L）. It was inoculated in five different M. sativa cultivars by greenhouse test. The results showed that the salt tolerant strains can product nodules in five M. sativa cultivars which indicated the diversity of symbiotic effect. In addition, it can promote growth of five inoculated M. sativa cultivars. Total nodules number were increased 11.4%-77.7%,effective nodules weight were increased 26.4%-80.8% ,fresh aboveground biomass weight were increased 45.8%-87.5 %, dry aboveground biomass weight were increased 49.1%- 106.3%, and crude protein were increased 1.5 %- 13.5 % after inoculation. Golden Empress fresh aboveground biomass weight, dry aboveground biomass weight and crude protein were increased 82.6%, 106.3 and 13.5%, respectively. Which were significant more than CK.
出处
《草业学报》
CSCD
2008年第5期43-47,共5页
Acta Prataculturae Sinica
基金
国家重点基础研究发展规划项目(2007CB108903)资助
关键词
紫花苜蓿
耐盐根瘤菌
接种效果
Medicago sativa
saline tolerance rhizobium
inoculation effect
