摘要
目的探讨正畸力对牙周组织胰岛素样生长因子(IGF)表达的影响,从体内实验角度阐明雌激素和应力对局部骨改建的调控途径和机制。方法建立在Wistar雌性大鼠动情周期不同阶段给予正畸加力的实验模型,采用核酸原位杂交技术检测雌性大鼠动情周期不同阶段牙周组织中IGF mRNA的表达变化。采用SPSS11.0软件包,单因素方差分析法(one way ANOVA)比较动情周期同一阶段加力实验组与对照组差异,Student-Newman-Keuls(S-N-K)法进行同一组内动情周期不同阶段各亚组间的两两比较。结果正畸牙移动使处于动情周期不同阶段的雌性大鼠牙周组织中的IGF mRNA含量增加,其中IGF-ⅠmRNA的表达在动情期最低,而动情前期最高(P<0.05);而IGF-ⅡmRNA的表达在动情周期中没有明显规律。结论IGF-Ⅰ不仅可以在应力反应中由骨形成细胞在局部合成,而且还与动情周期的全身激素状态有关,而IGF-Ⅱ则是对应力发生反应的一种局部生长因子。
Objective To elucidate the regulatory bio-mechanism of estrogen and local stress on the bone remodeling activities in vivo. Methods A Wistar rat experimental model was established to apply orthodontic activation during different stages of estrous cycle. In situ hybridization was used to detect the variation of the insulin-like growth factors （IGF） mRNA expression in the periodontal tissues. The data of each group was analyzed with SPSS 11.0 software package for Student-Newman-Keuls （S-N-K） test and one way ANOVA test. Results Both IGF- I mRNA and IGF-Ⅱ mRNA level expressed in the periodontal tissues were increased by orthodontic activation during various stages of the estrous cycle. But a similar pattern emerged that the IGF- I mRNA level was the lowest in the estrous stage, whereas its level was the highest in the proestrus stage （P〈0.05）. No rhythm of IGF-Ⅱ mRNA expression found during the estrous cycle. Conclusion It was concluded that local mechanical stimulation directly enhanced the IGF expression. In contrast to the IGF-Ⅱ, IGF-I was also influenced by the sex hormone rhythm during the estrous cycle.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第4期439-442,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30371560)
关键词
牙移动
动情周期
胰岛素样生长因子
tooth movement
estrous cycle
insulin-like growth factor
