摘要
以乳酸乳酸球菌７９ＵＶＳ２２为出发菌株，用紫外线、ＤＥＳ、ＬｉＣｌ等多种理化因子进行诱变处理，获得一株乳链菌肽高产突变株ＤＬ２０３，其效价稳定在２０００ＩＵ／ｍｌ以上。对其生理特性的研究表明，ＤＬ２０３菌的生长及发酵最适温度为３０℃，最适ｐＨ值为７．９，通风可以促进菌体的生长，但对乳链菌肽的产生无影响。
A mutant strain DL-203 with high yield of Nisin is obtained from Lactococcus Lactis subsp.Lactis 79UV-S22 treated by different physicochemical factors. The Nisin activities of fermentation medium are higher than 2000 IU/ml. The optimum pH value and temperature for Nisin production are 7.9 and 30°C, respectively. Ventilation increases the cell mass greatly, but exerts little influence on the Nisin production.
出处
《大连轻工业学院学报》
1997年第4期57-61,共5页
Journal of Dalian Institute of Light Industry
关键词
乳酸乳酸球菌
乳链菌肽
诱变育种
NISIN
lactococcus lactis subsp.lactis
nisin
mutation breeding
physiological character
