摘要
目的建立基于16S rRNA基因的PCR细菌学检测方法,用于慢性非细菌性前列腺炎患者前列腺液的细菌学检查。方法应用PCR方法检测105例慢性非细菌性前列腺炎患者的前列腺液中细菌16S rRNA基因。用PCR技术扩增10株实验室保留株的16S rRNA基因,以HBV-DNA、白假丝酵母菌和人类基因组DNA为对照,检测该方法的特异性;采用10倍比稀释法进行该方法的灵敏度检测。结果细菌16S rRNA基因的检出率在前列腺液中为35.2%。对所测细菌株均获得371 bp扩增产物,而与HBV-DNA、白色假丝酵母菌和人类基因组DNA无交叉反应;PCR最低能检测15 CFU/mL大肠杆菌。结论PCR具有快速、特异性和敏感性高的特点,可用于慢性非细菌性前列腺炎患者的前列腺液中细菌16S rRNA基因的检出。
Objective It is to establish the detected methods with PCR base on bacterial 16S rRNA gene,in order to detect the EPS of patients with chronic abacterial prostatitis.Methods The bacterial 16S rRNA gene signals in EPS of 105 patients with chronic abacterial prostatitis were detected by PCR.The specifieness was detected by way of amplifing 16S rRNA gene of ten bacterial species with PCR,and comparing with HBV-DNA,candida albicans and human genome DNA.The sensitivity test was done by the method of 10 gradual dilution.Results Positive rate of bacterial 16S rRNA gene in EPS was 35.2%.The bacterial species were amplified and the products were 371bp in length,but HBV-DNA,candida albicans and human genome DNA showed no amplification products.Sensitivity test showed that it could detect as low as15 CFU/mL of E.coli.Conclusion The method of PCR is rapid and highly specific and sensitive in detecting the existence of bacterial 16S rRNA gene.It can be considered as one effective method to detect EPS of patients with chronic abacterial prostatitis.
出处
《现代中西医结合杂志》
CAS
2008年第30期4672-4673,4676,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
