摘要
目的探讨碱性成纤维细胞生长因子（ｂＦＧＦ）在慢性低氧性肺动脉高压中的作用。方法建立慢性低氧性肺动脉高压大鼠模型，测定肺血流动力学及肺小动脉显微形态计量学，用免疫组化方法观察ｂＦＧＦ在大鼠肺组织的表达和分布。结果缺氧组大鼠平均肺动脉压（ｍＰＡＰ）、肺小动脉壁厚度占血管外径的百分比（ＭＴ％）分别为３．９６±０．４７ｋＰａ、３４±４％，明显高于对照组（Ｐ＜０．０１）；肺小动脉壁ｂＦＧＦ染色明显强于对照组（Ｐ＜０．０１），并和ＭＴ％呈正相关。结论（１）慢性缺氧能导致肺小动脉重建及肺动脉高压。（２）ｂＦＧＦ参与了慢性低氧性肺动脉高压肺血管重建的调控。
Objective To evaluate the role of bFGF in the development of hypoxic pulmonary hypertension. Method Rat models with chronic hypoxia induced pulmonary hypertension were established,the pulmonary hemodynamics were measured and the pulmonary arterioles change were studied with morphometric analysis under light microscopes,immunohistochemical staining with monoclonal antibody against human recombinant bFGF was performed in the paraffin section of rat lung. Result (1) The mean pulmonary artery pressure (mPAP),and the ratio of the thickness of pulmonary arteriolar wall to external diameter of pulmonary arterioles (MT%) were 3.96±0.47kPa and 33.8%±3.5% in rats exposed to hypoxia for 3 weeks respectively,both were significant higher than those in normal control group,P<0.01.(2) The positive staining for bFGF in the wall of pulmonary arterioles in hypoxic rats was stronger than that of control group (P<0.01), there was a statistical relationship between increase of staining for bFGF and MT% in rats exposed to hypoxia. Conclusion (1)Hypoxia can induce formation of pulmonary hypertension and structual remodeling of pulmonary arterioles.(2)bFGF may modulate the structure remodeling of pulmonary arterioles in chronic hypoxic pulmonary hypertension.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1997年第6期344-346,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家"九五"攻关基金
