摘要
１５只家兔的颈动脉体窦神经标本，记录了３３个窦神经中化学感受性传入单位的电活动。实验结果：①大部分单位放电频率较低且不规则，少部分单位的放电频率高而规则；②在灌流液内加入乙酰胆碱（ＡＣｈ）２０μｇ／ｍｌ后，１６个单位的自发放电频率由１．２６±０．３１ｉｍｐ／ｓ减至０．７７±０．１９ｉｍｐ／ｓ（Ｐ＜０．０５）。③在灌流液中加入尼古丁（１、３、６、１０、和１５μｇ／ｍｌ）后，１０个单位的自发放电频率，由０．６０±０．２１ｉｍｐ／ｓ分别增加到０．９６±０．２１、１．１９±０．３０、１．２４±０．２９、１．４８±０．４１和１．８２±０．３９ｉｍｐ／ｓ，有明显的剂量依赖性（ｒ＝０．９４，Ｐ＜０．００１）。④阿托品（１０μｇ／ｍｌ）可阻断ＡＣｈ的作用，筒箭毒（１．０μｇ／ｍｌ）可阻断尼古丁作用，但对自发放电均无显著影响。上述结果提示，ＡＣｈ在颈动脉体化学感受性功能方面可能仅起调质的作用。
The unit discharge of carotid chemosensory afferents were recorded in the carotid body(CB)-sinus nerve preparations from 15 rabbits in vitro(n=33).The results were as follows:①The discharge characteristics of chemoreceptor afferent units examined were generally that most of the units had a lower (<2imp/s)irregular frequency,but the other units(<10%) had a higher (>2imp/s) regular one.②The perfusate containing acetylcholine(ACh)20μg/ml decreased the discharge frequency from 1.26 ± 0.31 to 0.77 ± 0.19 imp/s( n=16 ,P<0.05).③Adding nicotine in the doses of 1,3,6,10 and 15μg/ml to the perfusate led to dose dependent increase in the discharge from 0.60±0.21 to 0.96 ± 0.21 , 1.19 ± 0.30 , 1.24 ± 0.29 , 1.48 ± 0.41 and 1.82±0.39 imp/s,respectively(r=0.94,P< 0.001 ,n=10).④Atropine(10μg/ml) antagonized the effect of ACh,but had no significant influence on the spontaneous discharge.Nicotinic stimulation was antagonized by D-tubocurarine(1.0μg/ml),while the basal discharge was not significantly affected.The results suggest that the possible physiological role of ACh may only act as a modulator on carotid chemosensory activity.
出处
《河北医科大学学报》
CAS
1997年第6期330-333,共4页
Journal of Hebei Medical University
基金
国家自然科学基金
