摘要
目的研究重组人促红细胞生成素（rhEPO）对糖尿病肾病患者内皮祖细胞（EPCs）数量与功能的影响，并探讨其作用机制。方法以不同浓度（0．3、0.6、1．2kU／L）rhEPO分别作用于20例健康人（对照组）及20例糖尿病肾病患者（DN组）EPCs，二苯基四氮唑溴盐（MTT）法检测其增殖，Annexin—V碘化丙啶法检测其凋亡，Westernblot法检测Akt蛋白激酶的表达。结果不同浓度rhEPO均能提高对照组和DN组EPCs的数量及增殖能力，呈剂量依赖性，但对DN组作用更显著。1．2kU／LrhEPO能显著降低DN组EPCs的凋亡率，提高Akt蛋白激酶的表达。加入Wortmannin后能阻断这一效应。结论rhEPO能提高糖尿病肾病患者EPCs的数量与功能，且这一效应与PI3K／Akt信号途径有关。
Objective To study the effect and possible role of recombinant human erythropoietin （rhEPO） on the proliferation and apoptosis of endothelial progenitor ceils （EPCs） from patients with diabetic nephropathy （DN）. Methods Various concentration of rhEPO was added to the culture system of EPCs from 20 DN patients （DN group） and 20 normal people （control group）. MTT assays were used to detect the proliferative rate, Annexin V/PI stains to detect the apoptotic rate, and Western blot assays to detect the expression level of Akt protein kinase. Results Proliferative ability of EPCs from control group and DN group was improved when concentration of rhEPO was 0.3, 0.6 and 1.2 kU/L, and it was dose-dependent. The effect i^m the latter was more obvious. The apoptotic rate of DN group was lowered and the expression levels of Akt protein kinase were upregulated when the concentration of rhEPO was 1.2 kU/L, while this kind of effect was blocked after Wortmannin was added to the culture system. Conclusion rhEPO can improve the number and function of EPCs from both healthy volunteers and patients with DN. PI3K/Akt pathway may play an important role.
出处
《中国医师进修杂志（内科版）》
2008年第10期12-15,共4页
Chinese Journal of Postgraduates of Medicine
关键词
红细胞生成素
糖尿病肾病
内皮祖细胞
Erythropoietin
Diabetic nephropathies
Endothelial progenitor cells
