摘要
氯化卞法微量提取猪瘟病菌7个生理小种和马唐瘟病菌基因组DNA，用60个随机引物进行PCR扩增，其中21个引物有特异性扩增荣带，每一引物─菌系组合扩增到3～19条，平均10．3条；其中有多态性条带为1～12条，平均6．1条，多态性为61．1％。说明稻瘟病菌群体有丰富的RAPD多态性。引物OPH1，OPH2，OPH4，OPH13，OPH14，OPK14等扩增的条带多，多态性丰富，而且可以有效地区分稻瘟病菌小种间的差异。几乎所有21个引物都能区别稻瘟病菌与马唐瘟病菌间的差异，而且有些引物如OPG3，OPG8，OPG13，OPK1等所扩增的产物表现为，稻瘟病菌生理小种间条带少，多态性差，而马唐瘟的条带多。说明两者在遗传上存在较大差异。
The genomic DNA of 7 rice physiological races and 1 weed [Digitaria sanguinalis(L.) Scop. jisolote of Pyricularia grlsea were obtained by using benzyl chloride.The Random Amplified Polymorphic DNA (RAPD)analysis was conducted with 21 effecti ve primers of 60 random decamer primers tested. Three to nineteen bands, averaging 10. 3, were amplified for each primer -isolate combination with 1- 12 polymorphic markers, averaging 6. 1, revealing that rice blast fungus was abundant in RAPD. Numerous bands and polymorphic markers were produced by such primers as OPH 1, OPH2, OPH4,OPH 13, OPH 14 and OPK 14, and the difference between the races of rice blast was easily identified. The difference of the fungi between rice blast and Digitaria b1ast could be identified by almost all of the 21 primers, and the products amplified by some primers,such as OPG3, OPG 13 and OPK 1, showed very few bands in the rice races but abundant in the Digitaria isolate, suggesting thcir genetic diversity.
出处
《热带作物学报》
CSCD
1997年第2期92-97,共6页
Chinese Journal of Tropical Crops
基金
福建省教委资助
关键词
稻瘟病菌
生理小种
遗传标记
群体生理学
Pyricularia grisea
physiologic races
RAPD
population biology
