摘要
以玉米(Zea mays L.)根尖为材料,研究孔雀石绿对玉米根尖的致畸效应。以不同浓度的孔雀石绿为诱变剂,测定玉米根尖细胞的微核率、有丝分裂指数和染色体畸变率。结果表明:孔雀石绿能诱发较高频率的微核率,即在一定的浓度范围内(0mg/L～0.50mg/L)其微核率随孔雀石绿处理浓度的升高而增加,但高于一定浓度(0.50mg/L～2.00mg/L)反而呈下降趋势;在一定浓度时能引起玉米分生组织细胞的分裂指数变化,在1.00mg/L时,有丝分裂指数最大;孔雀石绿还能诱导玉米根尖细胞产生较高频率的染色体畸变,在0.50mg/L时,畸变率最高且产生多种类型的染色体畸变。孔雀石绿对玉米根尖细胞具有明显的致畸效应,在一定范围内可以指示环境中孔雀石绿的污染程度。
The aberrant effects of malachite greens at different concentrations were investigated with Zea mays L. root tip cells. The micronucleus, mitosis and chromosome aberration assay were conducted to determine the micronucleus frequencies, mitotic index and chromosome aberration frequencies of Zea mays L. root tip cells induced by malachite greens. The results showed that the malachite green could increase the micronucleus frequencies of Zea mays L. root tip ceils. With certain range of concentrations, （0-0.50）mg/L, the frequencies of mierenucleus were found to be increased with the increase of malachite green concentration. But in the range of （0.50-2.00）mg/L, the frequencies of micronucleus decreased with the further increase of malachite green concentrations. The malachite green at different concentrations could affect the cell mitosis index, and the maximum index appeared at the concentration of -1.00 mg/L. It also caused various types of chromosome aberration, and the frequencies of chromosome aberration were always higher than those of the control group and the maximum frequency appeared at 0.50 mg/L. The conclusion of this study was that malachite green has obvious cytogenic effects on Zea mays L. root tip cells. In some degree the micronucleus frequencies of plant cells was able to indicate the pollution grades of malachite green in the environment.
出处
《中国测试技术》
2008年第5期89-91,共3页
CHINA MEASUREMENT & TESTING TECHNOLOGY
关键词
孔雀石绿
玉米根尖细胞
微核
染色体畸变
有丝分裂指数
Malachite green
Zea mays L.
Mieronucleus frequency
Chromosome aberration
Mitotic index
