摘要
[目的]从基因水平探索肌萎缩侧索硬化(ALS)其发病机制并发现治疗ALS的新靶点。[方法]通过GeneSifter软件对野生型(WT)和重度联合免疫缺陷型(SCID)小鼠肺组织钩虫感染的微阵列表达谱进行分析,使用two-way ANOVA的统计学方法对数据聚类,并结合Gene Ontology和KEGG(Kyoto Encyclopedia of Genes and Genomes)生物学通路,深入分析具有种系效应的517个差异基因(P﹤0.001)。[结果]显示ALS的信号转导通路在两组小鼠间差异有统计学意义(Z﹥2),与Th2反应相关的多个基因对ALS的代谢异常具有保护作用。[结论]筛选出的3个特异基因,可作为治疗肌萎缩侧索硬化的新候选基因。
[Objective] To investigate the molecular pathogenesis of amyotrophic lateral sclerosis {ALS} at genetic level and so as to develop the new targets to cure it. [ Methods ] Methods Used two-way ANOVA, Gene Ontology and KEGG methods for the analysis of gene expression pattern of lung-stage Helminth infection in wild type and SCID mice with GeneSifter software, and then further investigated on 517 genes with strain-effect （P〈 0.001）. [Results] The pathway of amyotrophie lateral sclerosis {ALS} was significantly different between the two type of mice （Z 〉 2）, several genes relevant with Th2 had a positive effect on the abnormal metabolic process of ALS. [Conclusion] 3 novel genes selected to be candidates may have thera- peutic benefits for amyotrophie lateral sclerosis.
出处
《现代预防医学》
CAS
北大核心
2008年第19期3787-3789,共3页
Modern Preventive Medicine
基金
广东省重点实验室资助(960327)
