摘要
目的探讨长期经口暴露铜绿对大鼠健康的影响及其可能的作用机制。方法选用80只SPF级SD大鼠,雌雄各半,随机分为4组,铜绿染毒剂量分别为0、7.3、24.4和73.2mg/kg。每周大鼠经口灌胃染毒6天,第7天称重,连续13周,记录动物的外观体征、行为活动等。13周末处死动物,观察大鼠血清生化指标和尿液指标;取脑、心、肝、肾、胃和脾等脏器称重并对脏器进行病理学检查,同时测定组织匀浆中的丙二醛(MDA)水平、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力。结果大鼠的行为活动、摄食饮水、粪便尿液、组织超微结构正常,动物体重增加。与对照组比较,低剂量组雄性大鼠体重显著增加(P<0.05);高剂量组大鼠血清乳酸脱氢酶活性较对照组显著降低(P<0.05);实验组大鼠肝脏、脑、心脏SOD活力增加,心脏GSH-Px活性增强,与对照组比较,差异有统计学意义(P<0.05);中、高剂量组大鼠胃SOD、GSH-Px活性显著降低,MDA含量显著增高(P<0.05)。结论在一定的暴露剂量下,铜绿可明显增加大鼠体重,提高大鼠肝、心和脑的抗氧化酶的活性,但是过量的铜绿会对胃组织产生一定的氧化损伤。
Objective To study the 90-day subchronic health effects of patina,and its effects on anti-oxidative enzymes activities and lipid peroxidation levels in important organs of SD rats.Methods Eighty SD rats（40 males,40 females） were divided into control group（treated with sodium carboxymethycellulose） and patina exposure groups of different dose levels（7.3,24.4 and 73.2mg/kg bw）.Animals were administered once daily by gavage,6 times a week,continuously for 13 weeks.At the end of the study,clinical signs,body weights,serum biochemistry,urinalysis,organ weights were examined.Pathological examination,the activities of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,and the concentrations of malondialdehyde（MDA） were respectively determined in brain,heart,liver,kidney,spleen,stomach.Results During the test period,there were no abnormal changes in the behavior,dieting,drinking,urine indicator,pathological changes and the body weights increased.At the dose of 7.3mg/kg,significant increase was found in body weight of male rats（P〈0.05）.At the exposed group,the activities of LDH of serum in the 73.2mg/kg appeared a significant decrease comparing with control group（P〈0.05）,and there were significant increased in activities of SOD in livers,brains,hearts and activities of GSH-Px in hearts（P〈0.05）.Meanwhile,the stomach levels of SOD,GSH-Px were significant reduced and MDA were significant increased at the two higher doses（24.4 and 73.2 mg/kg patina）,compared with control group（P〈0.05）.Conclusion At certain levels,patina may increase body weight,improve the activities of anti-oxidative enzyme in brains,hearts,livers,but overload patina may cause oxidative damage to stomachs of rats.
出处
《卫生研究》
CAS
CSCD
北大核心
2008年第5期546-550,共5页
Journal of Hygiene Research
基金
国际铜业协会(ICA)资助课题(No.H-AS-07-03)
