摘要
作者用阿霉素(5mg/kg)给大鼠一次性静脉注射,造成功极似人类微小病变型肾病综合征的动物肾病模型,表现为严重蛋白尿、低白蛋白血症、高胆固醇血症及胸、腹腔积液;电镜下肾小球上皮细胞足突肿胀、融合,光镜下病变甚微;上皮细胞足突肿胀、融合先于白蛋尿出现。结合以前所作肾小球多阴离子定量分析的结果,认为多阴离子的丢失系上皮细胞足突肿胀、融合和白蛋尿产生的主要因素。该模型具有方法简便,蛋白尿持续时间长,病变稳定,药源充足,制作成功率高等优点。
A single intravenous injection of ad-riamycin (5mg/ kg) into rats causedthe full expression of nephrotic syndromecharacterized by heavy proteinuria, hy-poalbuminemia, hypercholesterolemia,thoracic and ascitic fluid, swelling andfusion of foot processes of epithelial cellscould be observed under electron micro-scope;histologic examination by light mi-croscopy did not reveal any significantchanges. The features of the model in cli-nic and pathology were very similar tothose described humans with minimalchange nephrotic sydrome. The observa-ation in the various periods of the modelindicated that the ultrastructure changesin epithelial cells occurred prior to theonset of proteinuria. The present study,combined with the results of quantitativeanalysis with image analyzer for altera-tion of glomerular polyanions. suggeststhat both morphologic changes and pro-teinuria may be the consequence of acommon primary event that is the loss ofglomerular polyanions. The model has theadvantages of being rather simple ndconvenient;providing lasting proteinuriaand pathological changes in stable condi-tion; and having high reproducibility anda sufficient suppy of the drug used.
出处
《华西医科大学学报》
CAS
CSCD
1990年第4期430-432,共3页
Journal of West China University of Medical Sciences
关键词
阿霉素
肾病
动物模型
大鼠
Adriamycin Animal model Nephrotic syndrome Rats
