摘要
目的研究非选择性环氧化酶(COX)抑制剂舒林酸对结肠癌细胞株HT-29生长的影响及其参与引起细胞死亡的可能机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测舒林酸对结肠癌细胞增殖的抑制,激光共聚焦显微镜(LSM)及荧光显微镜观察细胞凋亡,流式细胞仪(FCM)分析其对细胞凋亡及细胞周期的影响。结果MTT显示舒林酸能呈剂量和浓度依赖性抑制HT-29的增殖。TUNEL染色荧光显微镜下观察凋亡细胞呈棕褐色,Annexin V/PI染色后LSM观察凋亡细胞胞膜绿染,胞核红染或呈桔黄色,FCM显示此药促进细胞的凋亡,使处于G0/G1期的细胞比例显著降低。结论舒林酸可抑制结肠癌细胞株HT-29生长,促进其凋亡,其机制可能与其阻止细胞周期的进展有关。
Objective To investigate the influence of sulindac,a non-selective cyclooxygenase（COX） inhibitor,on the proliferation and apoptosis of colorectal cancer cell HT-29 and its mechanisms. Methods Cultured HT-29 cells were treated with sulindac. MTT assay and flow cytometry were used to measure the proliferation and apoptosis respectively. The laser scaning microscope （LSM） and the fluorescence microscope were used to observe apoptosis of the ceils,and the flow cytometry（FCM） was used to analyze its influence on the cell apoptosis and the cell cycle. Results Sulindac inhibited the cells proliferation and induced apoptosis in a dose and time-dependent manner. With the TUNEL staining and observing under the fluorescence microscope we found that the apoptsis cell became brown. After the AnnexinV/PI staining,we observed with LSM that the membrane of apptosis cells became green,its nucleosidase became red or crocus. FCM showed that this medicine promoted apptosis of the cells,made the cells in the stage of G0/G1 significantly reduced. Conclusion Sulindac may inhibit the proliferation and induce apoptosis of colon cancer cell HT-29,and its mechanism probably relates to the progress of its prevention on cell cycle.
出处
《重庆医学》
CAS
CSCD
2008年第15期1704-1705,1708,F0002,共4页
Chongqing medicine
关键词
姑肠癌细胞
舒林酸
细胞凋亡
colorectal adenoma cells
sulindac
apptosis
