摘要
目的探讨雄激素对大鼠脑缺血/再灌注(I/R)损伤后,脑内内皮型一氧化氮合酶(eNOS)和诱生型一氧化氮合酶(iNOS)表达的影响。方法将大鼠45只随机均分为去势组、雄激素组和假手术组,喂养2周后,各组再按照I/R后不同时间点分为6,24和72 h 3个亚组,共9组,每组均5只。采用W estern b lot检测不同处理组脑内顶叶皮层中eNOS和iNOS表达的变化。结果随着再灌注时间的延长,各组大鼠eNOS的表达水平均有下降的趋势;而去势组和假手术组大鼠iNOS表达的水平均有上升的趋势。与假手术组相比,再灌注各时间点雄激素处理组eNOS的水平显著升高(P<0.01),去势组大鼠eNOS的水平明显降低(P<0.05)。而iNOS的表达水平则呈现出相反的趋势:即雄激素处理组明显低于正常水平(P<0.05);而去势组大鼠则显著升高(P<0.05)。结论脑I/R后,在雄激素存在的情况下,eNOS的表达水平升高,而iNOS的表达水平降低;去势组eNOS的表达水平下降,而iNOS的表达水平上升,提示雄激素对脑I/R损伤可能具有保护作用。
AIM To explore the effects of different levels of testosterone on the expressions of eNOS and iNOS after focal cerebral ischemic reperfusion injury in rats. METHODS Forty-five SD rats were randomly evenly divided into 3 groups： testosterone treated group, orchiectomized group and the sham operation group. The 3 groups were again devided into 3 subgroups （5 rats each group） according to different time points at 6 h, 24 h and 72 h after ischemic reperfusion. Western blot was used to detect the expressions of eNOS and iNOS in the parietal cortex of the rats. RESULTS The expression of eNOS was decreasing as the reperfusion time prolonged in each group, while the level of iNOS was up-regulated in the orchiectomized and the sham control group. The eNOS level of the testosterone treated group was significantly higher than that of the control group （P 〈 0. 01 ）, while it was much lower in the orchiectomized group （ P 〈 0. 05 ） at each time points after reperfusion. But the expression pattern of iNOS was totally contrary： significant lower in the testosterone treated group （P 〈 0. 05） but much higher in the orchiectomized group （P 〈 0. 05）. CONCLUSION The changing course of eNOS and iNOS after ischemic reperfusion injury is in positive and negative correlation with the level of testosterone, respectively. Testosterone probably has some protective effect on cerebral ischemic reperfusion injury.
出处
《心脏杂志》
CAS
2008年第5期556-558,共3页
Chinese Heart Journal
