摘要
目的探讨沙利度胺对大鼠胶原诱导性关节炎(CIA)的治疗作用及可能机制。方法采用鸡Ⅱ型胶原于 SD 大鼠尾跟部多点皮内注射建立 CIA 动物模型。设立沙利度胺治疗组(沙利度胺10 mg/d,共4周)、CIA 对照组和空白对照组。每日观察大鼠关节炎症积分和足垫厚度。4周后处死动物.检测各组大鼠血清肿瘤坏死因子α(TNF-α)水平;行后足远端趾间关节病理切片,观察关节病理损伤指数。结果注射鸡Ⅱ型胶原后28d。大鼠的关节炎症积分和足垫厚度与空白对照组相比差异有显著性(P<0.001),造模成功。给予沙利度胺后,大鼠关节炎症积分和足垫厚度逐渐改善,4周时与 CIA 对照组相比差异有显著性(P<0.001);血清 TNF-α水平和关节病理损伤指数无明显下降,与 CIA 对照组相比差异无显著性(P>0.05)。结论沙利度胺对 CIA 有治疗作用,其治疗作用不是通过降低血 TNF-α水平实现的,不能延缓关节软骨和骨的破坏。
Objective To study the effect and possible mechanism of thalidomide in treating rat collagen-induced arthritis （CIA）. Methods Type II chicken collagen was injected into the tails of Spragne-Dewley rats to establish arthritis models. Rats were divided into thalidomide group （10 mg orally per day for 4 weeks）, CIA control group, and blank control group. Articular inflammatory index and joint pad thickness were used to evaluate the effect of treatment. Serum tumor necrosis factor-or （TNF-ot） level as well as articular pathology index were also measured. Results Four weeks after collagen injection, the articular inflammatory index and joint pad thickness in CIA control group were significantly higher than those in blank control group （P 〈 0.001） and improved significantly in thalidomide group （P 〈 0.001）, while serum TNF-α level and articular pathology index showed no statistically significant difference between thalidomide group and CIA control group. Conclusion Thalidomide is effective in improving inflammation of rat collagen-induced arthritis enroute mechanism other than TNF-α inhibition and it might not inhibit cartilage and bone
出处
《中华临床免疫和变态反应杂志》
2007年第1期27-30,共4页
Chinese Journal of Allergy & Clinical Immunology
关键词
关节炎
胶原性
肿瘤坏死因子-Α
沙利度胺
arthritis
collagen-induced
tumor necrosis factor-α
thalidomide damag
