摘要
基因枪法是是指用钨粉或金粉包裹外源DNA,而后依靠基因枪装置,利用高压氦气冲击波加速微弹去穿透植物细胞壁和细胞膜,使外源DNA进入植物细胞并整合到植物细胞染色体组中,从而达到稳定遗传和表达的目的。它多用于对农杆菌不敏感的物种和基因型,用外源DNA包被的钨粒对大豆茎尖分生组织进行轰击,获得大豆转基因植株,此后,这一技术便很快在大豆中得到了应用。虽然它有缺点,但可与其他转基因技术结合使用。农杆菌介导法和基因枪轰击法各有自己的优缺点,它们之间并不是相斥的M相反,通过结合两种方法的特性,互相取长补短,就有可能提高转化效率。
the gene gun with tungsten powder or powder parcels of exogenous DNA, and then rely on the gene gun devices, using high-pressure helium shock-accelerated plant bombs to penetrate the cell wall and cell membrane so that the foreign DNA into the cells of plants and integrated into the plant cell chromosome in the group, so as to achieve stability and the expression of genetic purpose. It used to Agrobacterium not more sensitive species and genotypes with exogenous DNA coated tungsten tablets on soybean shoot tip-Health Organization bombardment, was transgenic soybean plants, then, this technology will soon be in the soybean Application. Although it has flaws, but with the other combined use of transgenic technology. Agrobaeterium-mediated gene gun bombardment law and have their own advantages and disadvantages, and they are not Xiangehe the M On the eontrary, through a combination of the two methods of mutual complementarity, it is possible to improve the conversion efficiency.
出处
《科技信息》
2008年第17期312-313,共2页
Science & Technology Information
