摘要
对基因工程菌pHBM-End培养条件优化的研究结果表明:250mL三角瓶中装入50mLLB培养基(含有10μg/mLTet),按5%的接种量接种种龄为12h的液体种子,培养4h后加入终浓度为0.2%的木糖进行诱导,9h后上清液中酶活可达889U/mL,是出发菌株C-36(79.2U/mL)的11.22倍。
The culture conditions of recombinant strain pHBM-End were studied.The results showed that the optimal conditions of culture were：volume 50 mL,the seed age 12 h,the inoculation rate 5 %,the inducer concentration 0.2 %, and the induction time 9 h.Under the optimal culture condition,the supernatant enzyme activity of endoglucanase is up to 889 U/mL which is about 11.22 times of the initial strain（79.2 U/mL）.
出处
《中国饲料》
北大核心
2008年第18期14-17,共4页
China Feed
基金
四川省教育厅自然科学科研项目(2005A030)
