摘要
建立高效液相色谱法测定消咳宁片中盐酸麻黄碱含量的方法。采用Zorbaxeclipse ODS C18色谱柱,以甲醇-0.1%磷酸溶液（10∶90）为流动相,检测波长为207nm,柱温30℃,流速1.0mL·min^-1,进样量为10μL。盐酸麻黄碱在0.04066～0.3659μg（r=0.9999）范围内与峰面积有良好线性关系,平均回收率为101.0%,RSD为1.12%（n=6）。方法操作简便快速,误差小,重复性好,可用于消咳宁片的质量控制。
The method for determination of ephedrine hydrochloride in Xiaokening Tablets by RP-HPLC was established.The analysis was performed on Zorbax eclipse ODS C18 column.The mobile phase consisted of methanol-0.1% phosphoric acid solution（10∶90）.The flow rate was 1.0 mL·min^-1 with UV detection at 207nm.The injective volume was 10μL.The linear range of ephedrine hydrochloride was 0.04066～0.3659μg（r=0.9999）.The average recovery was 101.0% with RSD 1.12%（n=6）.The method is simple,accurate and reproducible.It can be used for quality control of Xiaokening Tablets.
出处
《中国药事》
CAS
2008年第9期815-816,822,共3页
Chinese Pharmaceutical Affairs
