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植物叶绿体遗传转化及研究进展 被引量：13

Advances in the Research of Plant Chloroplast Genetic Transformation
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摘要 叶绿体遗传转化与传统的细胞核遗传转化相比具有许多独特的优点:外源基因表达效率高,可同时转化及表达多个基因,由于通过母性遗传,因此没有基因沉默现象及位置效应,也不会造成外源DNA的扩散。目前已经有超过四十多个外源基因整合到烟草等作物的叶绿体基因组中,表现出理想的农艺性状或作为生物反应器表达高水平的生物疫苗。本文对植物叶绿体基因组转化技术的原理和优点,外源基因导入叶绿体基因组的方法,以及目前叶绿体转化研究的最新进展进行了综述,并对其应用进行了展望。 Chloroplast genetic engineering offers a number of unique advantages, including high-level transgene expression, multigene engineering in a single transformation event, transgene containment via maternal inheritance, lack ofgene silencing, position and pleiotropic effects and undesirable foreign DNA. More than 40 transgenes have been stably integrated and expressed via the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals. In this paper, the principles and advantages of the chloroplast genetic engineering, the methods used for chloroplast transformation, and the recent advances in the field were illustrated and the application of the technology in future was also discussed.
作者 钱雪艳 杨向东 郭东全 赵桂兰 王丕武
机构地区 吉林省农业科学院生物技术研究中心 吉林农业大学农学院
出处 《分子植物育种》 CAS CSCD 2008年第5期959-966,共8页 Molecular Plant Breeding
基金 国家863计划项目(2007AA10Z189 2006AA100104-17) 国家自然科学基金项目(30771355)资助
关键词 叶绿体 遗传转化 研究进展 Chloroplast, Chloroplast transformation, Advances
分类号 Q943.2 [生物学—植物学] Q943 [生物学—植物学]
  • 相关文献

参考文献61

  • 1Arlen P.A., Falconer R., Cherukumilli S., Cole A., Cole A.M.,Oishi K.K., and Daniell H., 2007, Field production and functional evaluation of chloroplast-derived interferon-alpha2b, Plant Biotechnol. J., 5(4): 511-525
  • 2Bogorad L., 2000, Engineering chloroplasts: an alternative sitefor foreign genes, proteins, reactions and products, Trends Biotechnol., 18(6): 257-263
  • 3Boynton J.E., Gillham N.W., Harris E.H., Hosler J.P., Johnson A. M., Jones A.R., Randolph-Anderson B.L., Robertson D., Klein T.M., Shark K.B., and Sanford J.C., 1988, Chloroplast transformation in chlamydomonas with high velocity microprojectiles, 240(4858): 1534-1538
  • 4Carter H., Hockenberry T.N., Svab Z., and Maliga P., 1993, Kanamycin resistance as a selectable marker for plastid transformation in tobacco, Mol. Gen. Genet., 241 (1-2): 49-561
  • 5Chakrabarti S.K., Lutz K.A., Lcrtwiriyawong B., Svab Z., and Maliga P., 2006, Expression of the cry9Aa2 B.t. gcnc in tobacco chloroplasts confers resistance to potato tuber moth, Trangcnic Res., 15(4): 481-488
  • 6Chebolu S., and Daniell H., 2007, Stable expression of Gal/GalNAc lectin of Entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis, Plant Biotechnol., 5(2): 230-239
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二级参考文献55

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共引文献109

同被引文献171

引证文献13

二级引证文献212

分子植物育种
2008年 第5期

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