摘要
利用基因组DNA的RAPD、ISSR与SRAP等3种分子标记技术,以日本芜菁品种作为外类群,对来自于温州不同地区具有代表性的10个盘菜品种进行品种鉴定与遗传多样性分析。10个RAPD引物共产生多态性条带70条,多态率为71.7%;12个ISSR引物共产生142条清晰带,其中多态性条带70条,多态率为49.3%;8个SRAP引物组合共产生105条谱带,其中多态性谱带78条,多态性比率为74.3%,表明品种间存在较高的多态性。用单个引物NAURP299、NAUISR43以及SRAP引物组合me1/em2,都可以将11个品种完全区分开来。基于3种标记的聚类分析结果表明,11个材料可以分为3大类,一定程度上能够揭示品种之间园艺学性状的相似性及亲缘关系远近。
Fingerprinting and genetic diversity analysis of 10 different Brassica campestris L. ssp. Rapifera landrace cultivars from Wenzhou and one turnip cultivar from Japan were conducted with RAPD, ISSR and SRAP markers. 10 RAPD primers could produce 70 polymorphic bands, and the polymorphc rate was 71.7%; 12 ISSR primers produced 70 polymorphic bands of the total distinct 142 bands, and the polymorphic rate was 49.3%; 8 SRAP primer combinations generated 105 bands and the polymorphic rate was 74.3%. It was indicated there existed higher polymorphism among 11 cultivars. The primers of NAURP299, NAUISR43 and the SRAP combination of mel/em2 could discriminate all the 11 cultivars effectively. According to the result of clustering based on these marker analyses, 11 cultivars can be grouped into three clusters, which is in accordance with the horticultural taxomy and relationships among all cultivars.
出处
《分子植物育种》
CAS
CSCD
2008年第5期899-904,共6页
Molecular Plant Breeding
基金
温州科技发展项目(N2005B003)资助
关键词
温州盘菜
地方品种
指纹图谱
分子标记
Brassica campestris L. ssp. Rapifera, Landrace cultivar, Fingerprinting, Molecular marker
