摘要
目的在荷瘤动物模型观察小鼠肝癌细胞(H22)来源的外泌体(exosomes)激发宿主抗肝癌免疫应答的效应。方法用自行分离纯化的小鼠肝癌细胞H22源外泌体免疫小鼠,然后给小鼠皮下注射H22肿瘤细胞,观察肿瘤的生长状况。用MTT法检测小鼠脾细胞增殖情况和细胞毒活性;用免疫组织化学法检测肿瘤组织中CD4+、CD8+T淋巴细胞浸润情况。结果该外泌体使肿瘤出现时间延迟,肿瘤生长缓慢,小鼠生存率提高。促进脾淋巴细胞增殖并增强其细胞毒活性,促进CD4+和CD8+T细胞活化。结论小鼠肝癌细胞(H22)源外泌体能诱导抗肿瘤应答,有效地诱导特异性的杀伤肿瘤细胞活性,有针对亲本细胞的免疫保护作用。
Objective To investigate the induction of antitumor response H22 cell-derived exosomes. Methods Fifteen BALB/c mice were immunized and its immune mechanism of by H22 cell-derived exosomes three times at weekly intervals, then inoculated with the wild-type H22. Another 15 BALB/c mice that were injected with PBS were used as controls. In the 80-day observation period, the tumor growth and survival time were observed. MTF was used to determine lymphocyte proliferation and specific cytotoxicity of mice splenic cells. Immunohistochemical staining of CD4^＋ and CD8^＋ was performed for lymphocyte infiltration in tumor tissues. Results Immunization with exosomes derived from H22 delayed the tumor growth and promoted the survival rate of the mice bearing the tumor, and also induced lymphocyte proliferation and triggered strong specific cytotoxic response of spleen lymphocytes and activated CD4^＋ and CD8^＋T cells. Conclusion Vaccination of H22 cell-derived exosomes is effective in inducing the protective immunity. Furthermore, those exosomes have strong antitumor activities.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第19期1836-1838,共3页
Journal of Third Military Medical University
关键词
外泌体
肝细胞癌
抗肿瘤免疫
肿瘤疫苗
exosomes
hepatocellular carcinoma
antitumor immunity
tumor vaccine
