摘要
目的采用血管紧张素Ⅱ(angiotensin Ⅱ,AngⅡ)作为诱导剂,体外诱导成人脂肪间充质干细胞(adipose-de-rived mesenchymal stem cells,ADMSCs)向心肌细胞方向分化。方法自成人脂肪组织中分离培养ADMSCs,分别用0.05、0.10和0.20μmol/L3种不同浓度的AngⅡ诱导ADMSCs,同时设立对照组,倒置相差显微镜及透射电子显微镜下观察细胞的形态学特征,免疫细胞化学染色方法检测心肌特异性肌钙蛋白-I(cardiac troponin-I,cTn-I),计算转化率。结果诱导后第3周时,0.20μmol/L组可见cTn-I阳性细胞表达,第4周时0.10μmol/L组和0.20μmol/L组均有cTn-I阳性细胞表达,但0.20μmol/L组的阳性细胞数明显多于前组,且细胞形态变化显著。结论AngⅡ在体外可以诱导ADMSCs分化为心肌样细胞,其诱导分化的最佳浓度为0.20μmol/L。
Objective To study the effects of angiotensin Ⅱ （ Ang Ⅱ ）, as inducing agent in vitro, in inducing adult adipose-derived mesenchymal stem cells （ADMSCs） to the direction of myocardial cell. Methods ADMSCs were isolated and cultured from adult adipose tissues. Different concentrations of Ang Ⅱ （0.05, 0. 10 and 0.20 μmol/L, respectively） were used to induce ADMSCs. Untreated ADMSCs were used as normal control. The cell morphological feature was observed under inverted phase-contrast microscope and transmission electron microscope. Immunocytochemical staining was used to detect the cardiac troponin-Ⅰ （cTn-Ⅰ） and calculate the conversion rate. Results After three weeks of induction, cTn-Ⅰ-positive cells could be detected in 0. 20 μmol/L Ang Ⅱ group. Four weeks later, cTn-Ⅰ-positive cells were detected in 0. 10 and 0.20 μmol/L Ang Ⅱ groups, furthermore, the number of cTn-Ⅰ-positive cells in 0.20 μmol/L Ang Ⅱ group was significantly higher than that of other groups. Conclusion Ang Ⅱ can induce in vitro ADMSCs to differentiate into cardiocyte-like cells, and the best induction concentration is 0. 20 μmol/L.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第19期1795-1798,共4页
Journal of Third Military Medical University
基金
全军医学科研"十一五"计划面上项目(06MB094)~~
关键词
干细胞
脂肪组织
血管紧张素Ⅱ
心肌细胞
诱导分化
stem cells
adipose tissue
angiotensin Ⅱ
myocardial cells
induced differentiation
