摘要
通过对两种猪凝血酶原粗品制备方法（氢氧化镁吸附法和柠檬酸钡吸附法）的比较研究，发现钡法工艺的得率和纯度较高。本文着重研究柠檬酸钡、硫酸铵、磷酸二氢钾和Ｔｒｉｓ－ＨＣｌ等对凝血酶原的稳定和激活作用，并通过ＳｅｐｈａｄｅｘＧ－７５凝胶柱色谱估测活化产物猪凝血酶的分子量。
Two adsorption methods with Mg(OH) 2 or barium citrate to prepare crude prothrombin from pig blood were compared,adsorption.It was showed that the yield and purity of the barium citrate process were higher.The effect of the usual purifying reagents of barium citrate,(NH 4) 2SO 4,KH 2PO 4 and Tris HCl on stability and activity of prothrombin was also discussed.The molecular weight of pig thrombin was estimated through chromatography with Sephadex G 75.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1997年第10期435-438,共4页
Chinese Journal of Pharmaceuticals
关键词
凝血酶原
猪血
制备
prothrombin,pig blood,preparation,property
