摘要
本文研究了柞蚕核多角体病毒的分离、纯化、纯化后的病毒DNA经限制性内切酶Pat Ⅰ、Hind Ⅲ、BamH Ⅰ、XhoⅠ、SalⅠ、EcoRⅠ及EcoRⅠ+BamHⅠ等酶解后电泳分别形成31、26、6、15、24、5及7条区带。据内切酶图谱测算柞蚕核多角体病毒的分子量为75.16±2.3×10~6道尔顿。并比较了柞蚕、蓖麻蚕及家蚕等核多角体病毒的亲缘关系。
Viral DNA was isolated from the nuclear polyhedrosis virus of the Chinese oak silkworm, Antheraea pernyi. The results of restriction endonuclease digestion revealed that PstI, Hind-III, XhoI, SalI, EcoRI and EcoRI+BamHI cleaved the ApNPV DNA into 31, 25, 6, 15, 24, 5 and 7 fragments, respectively. By lambda DNA fragments as standard, the mean molecular weight of ApNPV DNA obtained by summation of the molecular weights of the fragments was about 75.15±2.3×106 daltons. Comparison betweea the DNA of APNPV and Plisamia cynthia ricini NPV and Bombyx mori NPV.
出处
《华南农业大学学报》
CAS
CSCD
1990年第3期1-5,共5页
Journal of South China Agricultural University
关键词
柞蚕
核多角体
病毒
基因
DNA
Antheraea pernyl, Nuclear polyhedrosis virus, Restriction endonuclease pattern
