摘要
本文采用压缩空气吹展ＤＮＡ的制样方法，使得ＤＮＡ链在云母表面大致向着同一方向充分舒展，为ＤＮＡ线度的ＡＦＭ测量打下了基础。对λＤＮＡＨｉｎｄⅢ依次进行了酸变性、复性、ＤＮａｓｅⅠ降解处理，并分离一种抗内切酶降解的组分。ＡＦＭ研究表明该组分可能是一种新的多链ＤＮＡ。
Abstract In this paper,DNA sample for AFM imaging is prepared by blowing the solution with compressed air to fully stretch the DNA strands on the surface of mica,which lay a foundation for measuring the dimensions of DNA.λ DNA Hind Ⅲ is treated sequentially with acid denaturation,renaturation and DNase I digestion,and one component resisting digestion has been separated from the mixture.AFM studies reveal that this component may be a new kind of multi stranded DNA.
出处
《电子显微学报》
CAS
CSCD
1997年第6期706-709,共4页
Journal of Chinese Electron Microscopy Society
