摘要
目的探讨阿是止泻胶囊对甲状腺功能亢进（简称甲亢）性腹泻大鼠血浆胃肠激素的影响。方法8周龄雄性SD大鼠120只．按体质量随机分为对照组（10只）和甲亢性腹泻组（110只）。对照组按每只大鼠1ml／d生理盐水灌胃；甲亢性腹泻组给予生理盐水配制成40以的甲状腺片混悬液，每只大鼠1ml／d，灌胃给药。3周后尾静脉取血，测血清FT3，FT4根据大鼠血清FT3、FT4水平、体质量和湿粪的数量筛选出42只甲亢性腹泻大鼠。取40只甲亢性腹泻大鼠按体质量随机分为5组，每组各8只。空白组：按1ml／d生理盐水给药；阳性对照组：黄连素片按1．94g·kg^-1·d^-1给药；低、中、高剂量组：阿是止泻胶囊分别按0．63、1．26、2．52g·kg^-1·d^-1给药。各组连续灌胃7d，在给药前和给药后，静脉采血，分离血浆，采用放射免疫分析方法，观察阿是止泻胶囊对甲亢性腹泻大鼠血浆胃动素（MTL）、胃泌素（GAS）、血管活性肠肽（VIP）、生长抑素（SS）的影响。结果①甲亢性腹泻大鼠体质量[（344．0±12．9）g]降低，n[（4．58±0．70）mol／L]、FT4[（23．44±4．40）mol／L]升高，湿粪质量[（17．4±3．2）g]增加，与对照组[（386．0±1．8）g、（2．08±0．10）moL／L、（10．18±2．00）mol／L、（9．1±0．6）g]比较，差异有统计学意义（t值分别为6．85、9．80、7．66、7．18，P〈0．01）。②治疗后MTL高剂量组[（80．54±3．80）ng／L]和阳性对照组[（90．63±9．99）ng／L]较治疗前[（204．27±17．69）、（187．79±13．32）ng／L]降低，二者比较差异有统计学意义（t值分别为8．60、4．57，P〈0．01）。③除空白组外，治疗后GAS阳性对照组[（56．06±6．36）ng／L]、低剂量组[（90．88±4．18）ng／L]、中剂量组[（75．64±7．09）ng／L]、高剂量组[（44．32±3．72）ng／L]均较治疗前[（192．75±11．80）、（193．09±3．81）、（190．60±9．31）、（196．33±18．13）ng／L]降低，二者比较差异有统计学意义（t值分别为15．27、7．62、13．43、13．22，P〈0．01）；治疗后大鼠MTL、GAS、VIP各组间比较，差异均有统计学意义（F值分别为166．68、1503．53、216．68，P〈0．01）。结论阿是止泻胶囊能显著降低血浆中MTL、GAS水平、并提高VIP水平。
Objective Investigate the Asi-antidiarrheal capsule＇s effect on gastrointestinal hormones in blood plasma of thyroid hormone-induced diarrheic （Abbreviation： Hyperthyroid Diarrhea, HD） rats. Methods One hundred and twenty SD male rats about 8 weeks old were randomly divided according to their constitution into control group of 10 rats and thyroid hormone-induced diarrheic group of 1l0 rats. The control group rats were lavaged with isotonic Na chloride 1 ml/d. Thyroid tablets were made with isotonic Na chloride into 40 g/L suspl. The such solution with 1 ml/d was intragastrically administered to each rat in thyroid hormone-induced diarrheic group. After three weeks, blood was sampled from vena caudalis of each rat, FT3, FT4 were then detected in blood serum. Fourty-two thyroid hormone-induced diarrheic rats were screened based on FT3 and FF4 level in blood serum, wet stool and body weight. Fourty thyroid hormone-induced diarrheic rats were stochastically re-divided into 5 groups with 8 in each. The physiological saline with 1 ml/d was given to blank group, 1.94 g·kg^-1·d^-1 Berberine capsule to positive control group, and 0.63,1.26,2.52 g·kg^-1·d^-1 to low-dose, moderate-dose and high-dose groups respectively. Intragastric administration of each group continued for 7 days. Venous blood was centrifuged before and after administration and underwent radioimmunoassay to observe the effect of Ashi-antidiarrheal capsule on motility （MTL）, gastrin （GAS）, somatostatin （SS）, vasoactive intestinal polypeptide （VIP） in blood plasma of thyroid hormoneinduced diarrheic rats. Results （1）Weight of thyroid hormone-induced diarrheic rats decreased[ （344.0 ± 12.9）g], FT3[ （4.58 ± 0.70）mol/L] and FT4[ （23.44 ± 4.40）mol/L] increased, and weight of wet stool[ （17.4 ± 3.2）g] increased. Compared to control group[ （386.0 ± 1.8）g, （2.08 ± 0.10）mol/L, （10.18 ± 2.00）mol/L, （9.1 ± 0.6）g], there was a statistical significance （t = 6.85,9.80,7.66,7.18, P 〈 0.01 ）. （2）After treatment, high-dose Ashiantidiarrheal group[ （80.54 ± 3.80）ng/L] and positive control group[ （90.63 ± 9.99）ng/L] blood plasma MTL, compared to pre-therapy [ （204.27 ± 17.69）, （187.79 ± 13.32）ng,/L] was decreased, there was a statistical significance （t = 8.60,4.57, P 〈 0.01 ）. （3）GAS contents had respectively decreased compared to pre-therapy [ （192.75 ± 11.80）, （193.09 ± 3.81）, （190.60 ± 9.31）, （196.33 ± 18.13）ng./L] in positive control group [（56.06 ± 6.36）ng/L], low- dose group[ （90.88 ± 4.18）ng/L], midst-dose group[ （75.64 ± 7.09）ng/L] and high-dose group[ （44.32 ± 3.72） ng/L], except for blank group. There was a statistical significance （t =15.27,7.62,13.43,13.22, all P 〈 0.01 ）. The intra-group difference of MTL, GAS and VIP level had statistic significances before and after the treatment （F = 166.68,1503.53,216.68, P 〈 0.01 ）. Conclusion Ashi-antidiarrheal capsule can significantly lower the level of MTL and GAS in blood plasma, and raise the level of VIP.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2008年第5期499-502,共4页
Chinese Jouranl of Endemiology
基金
黑龙江省科委“十五”攻关课题（GC01C138-01）
