摘要
目的探讨p16基因在喉鳞癌中的表达及其临床意义。方法应用原位杂交法检测49例喉鳞癌组织、19例声带息肉、12例癌旁增生组织及8例非典型增生组织中p16基因的表达情况。结果喉鳞癌组织中p16基因表达呈阳性者5例,定位于胞浆,其余44例呈阴性表达。p16阳性表达例数在喉鳞癌组织、声带息肉、癌旁增生组织及非典型增生组织中分别为5,16,8,4例,喉鳞癌组织中p16mRNA的阳性表达显著低于声带息肉、癌旁增生组织及非典型增生组织(P<0.01),但与喉鳞癌的临床分期及患者性别无相关性(P>0.05)。结论喉鳞癌组织中存在p16基因的低表达;p16基因可能参与调控了喉鳞癌的发生过程。
Objective To explore the expression of p16 gene in laryngeal squamous cell carcinoma （ LSCC ） and its clinical significance. Methods In situ hybridization method was used to detect the expression of p16 gene in 49 LSCC tissues,19 vocal cord polyps,12 paracancerous tissues and 8 atypical hyperplasia tissues. Results Positive expression of p16 was seen in 5 cases of LSCC, which was located in cytoplasm. Positive p16 expression in the vocal cord polyps, paracancerous tissues and atypical hyperplasia tissues was seen in 5,16,8 and 4 cases respectively. The expression of p16 in the carcinoma tissues was significantly lower than that in the vocal cord polyps, paracancerous and atypical hyperplasia tissues（P 〈 0.05）, but was not correlated with gender and the clinical staging of LSCC （ P 〉 0.05 ）. Condu. sions There was low expression of p16 gene in LSCC, which suggested that p16 may participate in the regulation of tumorigenesis in LSCC. The presence of p16 gene may result in the development of LSCC. （ Chin J Ophthalmol and Otorhinolaryngol,2008,8：292-294）
出处
《中国眼耳鼻喉科杂志》
2008年第5期292-294,I0003,共4页
Chinese Journal of Ophthalmology and Otorhinolaryngology
关键词
基因
P16
喉肿瘤
原位杂交
Genes,p16
Laryngeal neoplasms
In situ hybridization
