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胃癌细胞C-myc蛋白对他莫昔芬的应答

Response of Protein C-myc to Tamoxifen in Grastric Cancer Cell
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摘要 目的:观察C-myc蛋白在胃癌细胞系BGC823中的表达情况,探讨胃癌细胞BGC823经他莫昔芬(TAM)处理后,C-myc蛋白表达的变化.方法:使用10 5MTAM处理胃癌BGC823细胞系24 h后,通过免疫荧光细胞化学的方法及细胞计数,检测C-myc蛋白的表达变化,并通过WST1法检测细胞活力的变化.结果:C-myc在胃癌BGC823中高表达,经10 5 M TAM处理24 h后BGC823细胞活力明显下降(P<0.05,)同时C-myc的表达减弱(P<0.05).结论:C-myc在胃癌细胞BGC823中发挥生物学作用且与TAM介导的BGC823细胞活力下降相关. Objective： To observe the expression of C-myc in gastric cancer BGC823 cell and research the alteration of C-myc expression in gastric cancer BGC823 cell after the treatment of Tamoxifen. Methods： By fluorescence immunocytochemistry staining and cell counting of BGC823 cell treated with 10^-5M Tamoxifen for 24 h, detected the expression alteration of C-myc, at the same time, cell energy was detected by WST-1 method. Results： The over expression of C-myc was detected in gastric cancer BGC823 cell. The decrease of expression of C-myc （P 〈 0.05） and cell energy （P 〈 0.05） were observed in gastric cancer BGC823 cell treated with 10^-5M Tamoxifen for 24 h. Conclusion： C-myc plays an important biological role in the gastric cancer BGC823 cell and is related to cell energy change mediated by Tamoxifen.
作者 余怡 曾智 邓昊
机构地区 江汉大学医学院 天津医科大学病理学系 江汉大学肿瘤研究所
出处 《江汉大学学报（自然科学版）》 2008年第3期73-75,共3页 Journal of Jianghan University：Natural Science Edition
基金 湖北省自然科学基金(2006AB191) 武汉市科技攻关计划引导项目(20055007170) 武汉市青年科技晨光计划项目(20045006071-7)
关键词 C—myc 胃癌 BGC823 他莫昔芬 C-myc gastric cancer BGC823 cell Tamoxifen
分类号 R735.2 [医药卫生—肿瘤]
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参考文献9

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二级参考文献20

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江汉大学学报（自然科学版）
2008年 第3期

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