期刊文献+

激光扫描共焦荧光显微镜的电子控制系统研究 被引量:1

Study on electronic control system for laser scanning confocal fluorescence microscope
在线阅读 下载PDF
导出
摘要 介绍了激光扫描共焦荧光显微镜的电子控制系统,为了提高激光扫描器的定位精度,在系统中提出采用基于闭环象素时钟发生器的检流计振镜所固有的扫描非线性失真补偿方案,减少输出图象的枕形畸变,实现行扫描空间位置的均匀性。在以细分梯形波电流驱动反应式步进电机情况下,根据实测θi曲线,用反插法求得与步进电机运行非均匀性相补偿的相电流波,从而获得在小电流情况下高角度均匀的步进电机细分运行特性。 The electronic control system for laser scanning confocal fluorescence microscope is described in this paper.For improvement of the positing precision of laser scanner,a high accuracy scanning image sampling method,based on pixels clock generator,is adapted to correct the non linear output image pincushion distortion caused by the rotational inertial of the e mechanical parts of laser scanner.In order to detect the scanning spatial position under the case of step motor driving,according to the experimentally measured I θ curve,we can get the phase current wave form which compensates the non linearity of I θ curve by means of interpolation method.Therefore,the step motor can operate with high angle uniformity under the condition of low current mode.
出处 《激光技术》 EI CAS CSCD 北大核心 1997年第5期284-287,共4页 Laser Technology
基金 国家自然科学基金
关键词 激光扫描 荧光显微镜 电子控制 帧扫描 行扫描 laser scanning electronic control system frame scan line scan
  • 相关文献

参考文献1

  • 1陈德,光学仪器,1994年,16卷,5/6期,1页

同被引文献14

  • 1李爱民,陶纯堪,刘明,陈龙岗,张慎,孙淑香,李连贵.共焦激光扫描显微镜的研制[J].激光技术,1994,18(5):261-263. 被引量:2
  • 2张平,吴震,王翠英,向际鹰.荧光共焦扫描系统成像特性的优化[J].光学学报,1997,17(3):308-313. 被引量:10
  • 3GU M.Principles of three-dimensional imaging in confocal microscopies [M].Singapare:World Scientific,1996.1~10.
  • 4DRAZIC V.Three-dimensional transfer function analysis of a confocal fluorescence microscope with a finite-sized source and detector [J].J Mod Opt,1993,40(5):879~887.
  • 5DRAZIC V.Three-dimensional transfer function of coherent confocal microscopes with extended source and detector [J].J Mod Opt,1992,39(8):1777~1790.
  • 6HELL S W,BOOTH M,WILMS S.Two-photon near- and far-field fluorescence microscopy with continuous-wave excitation [J].Opt Lett,1998,23(15):1238~1240.
  • 7CUMPSTEM B H,ANANTHAVEL S P,BARLOW S et al.Two-photon polymerization initiators for three-dimensional optical data storage andmicrofabrication [J].Nature,1999,398(4):51~54.
  • 8SUN H B,MATSUO S,MISAWA H.Three-dimensional photonic crystal strutures achieved with two-photon-absorption photopolymerization of resin [J].A P L,1999,74(6):786~788.
  • 9黄菁,梁瑞生,司徒达,张坤明,唐志列.高斯光束共焦扫描激光显微镜的光学传递函数[J].物理学报,1998,47(8):1289-1295. 被引量:6
  • 10唐志列,梁瑞生,朱小松,何振江,司徒达.偏振共焦扫描激光显微镜的成像特性研究[J].光学学报,1999,19(8):1118-1120. 被引量:14

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部