期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi技术研究进展及其基因治疗应用前景 被引量：5

Research Progress in RNA Interference and Its Application Prospect in Gene Therapy
暂未订购
导出
摘要 RNA干扰(RNAi)是一种由双链RNA介导的基因沉默现象。RNAi现象在各种生物中的普遍性引起了人们浓厚的兴趣。RNAi可能将成为基因治疗的新途径。 RNA interference（RNAi） is a phenomenon of gene silence induced by double-stranded RNA. Great interests are showed in the universality of RNAi in different organisms. RNA interference might become a new way to gene therapy.
作者 王欢 黄敏
机构地区 武警江西总队医院 江西省肿瘤医院
出处 《现代诊断与治疗》 CAS 2008年第5期291-295,共5页 Modern Diagnosis and Treatment
关键词 RNA干扰 双链RNA 基因沉默 RNA interference Double-stranded RNA Gene silence
分类号 R511 [医药卫生—内科学] R394 [医药卫生—医学遗传学]
  • 相关文献

参考文献32

  • 1Guo S, Kemphues KJ. Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed[ J]. Cell, 1995,81 (4) :611-620.
  • 2Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis [ J ]. Nature, 1998,391 (6669) :806-811.
  • 3Jorgensen RA, Culster PD, English J,et al. Chalcone synthase cosuppressiou phenotypes in petunia flowers:comparlson of sense vs antisense constructs and single-copy vs complex T-DNA sequences[J]. Plant Mol Biol,1996,31 (5) :957-973.
  • 4Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in mammalian cell culture[ J]. Nature, 2001,411:494-498.
  • 5McManus MT,Sharp PA. Gent silencing in mammals by small interfering RNAs [ J ]. Nature,2002,3 ( 10 ) :737-747.
  • 6Hamilton A J, Baulcombe DC. A species of small antisense RNA in posttranscriptional gene silencing in plants [ J ]. Science, 1999,286 ( 5441 ) : 950-952.
  • 7Zamore PD, Tuschl T, Sharp PA, et al. RNAi : double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals[ J]. Cell ,2000,101 ( 1 ) :25-33.
  • 8Bemstein E, Caudy AA, Hammond SM ,et al. Role for a bidentate ribonucleasc in the initiation step of RNA interference[ J]. Nature,2001,409 (6818) :363-366.
  • 9Lipardi C,Wei Q ,Paterson BM. et al. RNAi as random degradative PCR: siRNA primers convert mRNA into dsRNAs that are degraded to generate new siRNAs [ J ]. Cell ,2001,107 ( 3 ) :297-307.
  • 10Barstead R. Genome-wide RNAi [ J ]. Curr Opin Chem Biol, 2001,5 (1) :63-66.

二级参考文献32

  • 1Lee RC et al. Science, 2001,294 : 862-864.
  • 2Stark GR et al. Rev Biochem, 1998,67 : 227-264.
  • 3Yang S et al. Mol Cell Biol,2001,21(22) :7807-7816.
  • 4Holen T et al. Nucleic Acids Res,2002,30(8):1757-1766.
  • 5Brown D et al. TechNotes, 2002,9 (1) : 3-5.
  • 6Yu JY et al. Proc Natl Acad Sci USA, 2002,99 (9) : 6047-6052.
  • 7Svoboda Pet al . Biochem Biophys Res Commun, 2001,287(5):1099-1104.
  • 8Sui G et al. Proc Natl Acad Sci USA,2002,99(9): 5515-5520.
  • 9Lau GT et al. Biochem Biophys Res Commun, 2001,280(5) :378-1384.
  • 10Luo X et al. Mol Cell,2002,9(1):59-71.

共引文献32

同被引文献57

引证文献5

二级引证文献3

现代诊断与治疗
2008年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部