摘要
目的研究复方中药益气苓对自发性高血压大鼠(SHR)的抗氧化作用,探寻该复方中药改善高血压大鼠预后的作用靶点。方法将SHR大鼠随机分为益气苓组和对照组,检测两组血清超氧化物歧化酶(SOD),丙二醛(MDA)含量,尿8-异前列腺素F_(2α)(8-isoPCF_(2α))含量。并用大鼠高密度基因表达谱芯片对两组心肌细胞的基因表达进行分析,用RT-PCR及实时定量PCR方法对其中3条抗氧化相关基因的表达水平变化进行验证。结果复方中药益气苓组SHR尿中8-isoPGF_(2α)含量明显低于对照组,血清SOD含量高于对照组,MDA含量低于对照组,差异均具有显著性。芯片结果显示:复方中药益气苓组上调基因354个,下调基因121个。其中Hsphl,Hspca及Pam基因的RT-PCR和实时定量PCR验证与芯片结果相符合。结论该复方中药具有抗氧化作用。可能通过对Hsphl,Hspca及Pam等抗氧化相关基因的调控干预SHR的预后。
Objective To investigate the anti - oxidafion effects of compound Chinese medicine of Yiqiling, on the spon- taneously hypertensive rats（SHR） and to explore the new target for improving the prognosis of hypertension. Method SHR were randomly divided into 2 groups： Yiqiling group and the control group. The serum content of malondiadehyde （MDA） and superoxide dismutase（SOD）, and the urine content of 8- iso prostaglandin F2α（8- isoPGF2α） in two groups were measured. Examination of the gene expression profile was analyzed by using cDNA microarray. Three of the anti - oxidation genes were selected to undergo RT - PCR and quantitative real - time PCR. Results The 8 - isoPGF2α urine level and MDA serum level were lower, and SOD serum level was higher in Yiqiling group than those in the control group, the differences of the above indexes had statistical significance. In Yiqiling group the expression of 354 genes was up- regulated and that of 121 genes was down- regulated in comparison with the control group. The results of the Hsphl, Hspca and Pare genes detected by RT - PCR and quantitative real - time PCR were consistent with those detected by the microarray examination. Conclusion Yiqiling has anti- oxidation effects and improvement on the prognosis of SHR, and its possible mechanism is related with the regulation of Hsphl, Hspca and Pam genes expression.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第5期354-357,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
上海市科学技术委员会基金项目(034119917)。
