摘要
目的:比较老年人梅毒筛查阳性与确认指标之间的关系,分析各检测指标在老年梅毒诊断的临床价值。方法:用酶联免疫吸附试验及甲苯胺红试验作为梅毒筛查,对老年人阳性标本用梅毒螺旋体明胶凝集试验检测,两种方法均为阳性者用荧光梅毒螺旋体抗体吸收法(FTA-ABS)检测及蛋白质印迹法(WB)确认,并对实验结果进行统计分析。结果:对酶联免疫吸附试验筛查梅毒的76例老年人阳性标本,用梅毒螺旋体明胶凝集试验检测有72例阳性,阳性符合率94.7%;两种方法均为阳性的72例标本经FTA-ABS及WB检测确证阳性71例,阳性符合率98.6%;并且FTA-ABS检测与WB检测结果一致。结论:为了提高老年人梅毒的检出率及正确性,对梅毒筛查阳性标本,应用TPPA进行确证,有疑义者用FTA-ABS或WB进行进一步检测确证。
Objective：To compare the results by screening tests and confirmatory tests, and to evaluate the methods of detecting Treponema paUidum in the elderly. Methods：The positive samples detected by screening tests with enzyme-linked immunosorbent assay and syphilis toluidine red untreated serum test were respectively confirmed by treponema pallidum particle agglutination （TPPA）, fluorescent treponemal antibody absorption test（FTA-ABS） and Western blotting（WB） ,and then the results were analyzed statistically. Results：Seventy-two of the 76 samples were detected positive by TPPA in screening tests（94.7% ,72/76）. Of the 72 positive samples by TPPA,71 were simultaneously confirmed by FTA-ABS and WB assays （98. 6% ,71/72）. Condusiom：The positive samples of Treponema pallidum detected by screening tests in the elderly must be confirmed by TPPA assay and even by FTA-ABS and WB assays if needed.
出处
《蚌埠医学院学报》
CAS
2008年第5期599-601,共3页
Journal of Bengbu Medical College
关键词
梅毒螺旋体
明胶凝集试验
免疫印迹法
荧光梅毒螺旋体抗体吸收法
Treponema pallidum
particle agglutination
Western blotting
fluorescent treponemal antibody absorption test
