摘要
采用微管液相色谱法同时测定白术中的白术内酯Ⅲ、白术内酯Ⅰ和苍术酮。采用美国Micro-tech Ultra plusⅡTM微管柱通用型二元梯度高效液相色谱仪和Microsil C18微管色谱柱(150mm×1.0mm),以甲醇-乙腈-水(A相体积比为90∶5∶5;B相体积比为55∶5∶40)为流动相,梯度洗脱,流速50μL/min,在220nm波长下检测。在上述色谱条件下,3种组分分离良好,在相应的进样质量范围内具有良好的线性关系。白术内酯Ⅲ、Ⅰ和苍术酮的加标回收率分别为96.86%,97.13%和98.06%;相对标准偏差分别为1.63%,1.31%和0.39%。该法简便、快捷,能够用于白术的质量控制。
A microbore liquid chromatography（microbore LC）has been developed for the simultaneous determination of atractylenolide Ⅲ,atractylenolideⅠ and atractylon in Artactylodis macrocephala.In the method,a Microsil C18 column（150 mm×1.0 mm）was used with the mobile phase containing methanol-acetonitrile-water for gradient elution,the flow rate was 50 μL/min,and the detection wavelength was set at 220 nm.Under the optimized separation conditions,every component was separated thoroughly.The relationships between the concentrations and the peak areas of the three components were all linear.The recoveries were 96.86% for atractylenolide Ⅲ,97.13% for atractylenolideⅠand 98.06% for atractylon,the relative standard deviations（RSD）were 1.63%,1.31% and 0.39%,respectively.The method is simple,convenient,and can be used for the quality control of Artactylodis macrocephala.
出处
《色谱》
CAS
CSCD
北大核心
2008年第5期637-639,共3页
Chinese Journal of Chromatography
基金
国家"十五"攻关课题(2004BA721A21)
浙江省分析测试科技计划项目(2007F70067)
关键词
微管液相色谱法
白术内酯Ⅲ
白术内酯Ⅰ
苍术酮
白术
microbore liquid chromatography（microbore LC）
atractylenolide Ⅲ
atractylenolideⅠ
atractylon
Artactylodis macrocephala
