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利用76个微卫星标记分析滇南小耳猪的遗传多样性 被引量:7

Analysis of the Genetic Diversity of Diannan Small-ear Pig Using 76 Microsatellite Markers
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摘要 滇南小耳猪是云南省优良地方猪种之一,其数量大,分布广。为了阐明其群体遗传变异情况,以对其进一步有效保护和合理利用提供科学依据,本研究采用分布在家猪19条染色体上的76个微卫星标记对该猪群体54个个体进行了群体遗传变异检测。共检测到338个等位基因,每个座位的等位基因数在2~8个之间,有效等位基因数在1.9560~5.71432:间,平均每个座位等位基因数(4.4474±1.2044)个,有效等位基因数(3.5091±0.9197)个,群体平均杂合度及平均多态信息含量分别为0.6917±0.09797和0.6388±0.1142。研究结果表明,滇南小耳猪群体遗传多样性较丰富。 also one of provide Diannan small-ear pig is widely distributed in the various regions of Yunnan Province and is excellent indigenous breeds. In order to estimate the genetic variation in its population and guidance and basis genetic background for its conservation and further utilization, 54 individuals sampled from its natural population were examined by using 76 microsatellite loci located on swine's 19 chromosomes. A total of 338 alleles were detected, the number of alleles varied from 2 to 8 and the number of effective alleles from 1. 956 0 to 5. 714 3, and mean number of alleles and effective alleles at each locus were (4. 447 4± 1. 204 4) and (3. 509 1 ± 0. 919 7), respectively. The mean heterozygosity for 76 microsatellite loci was 0. 691 7±0. 097 9, whereas the mean polymorphism information content was 0. 638 8 ± 0. 114 2. The results revealed that the genetic diversity in the population of Diannan small-ear pig breed was high.
出处 《云南农业大学学报(自然科学版)》 CAS CSCD 2008年第5期638-643,共6页 Journal of Yunnan Agricultural University:Natural Science
基金 云南省教育厅科学研究基金项目(07Y40124) 国家自然科学基金项目(30660024) 云南省应用基础研究重点项目(2007C0003Z) 云南省应用基础研究计划面上项目(2006C0034M)
关键词 微卫星标记 滇南小耳猪 遗传多样性 杂合度 多态信息含量 microsatellite DNA Diannan small-ear pig genetic diversity heterozygosity polymorphism information content
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