期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

细胞外钙受体在妊娠期高血压疾病胎盘组织中的表达及意义 被引量：1

Expression and significance of calcium-sensing receptor in hypertensive disorder complicating pregnancy
原文传递
导出
摘要 目的探讨妊娠期高血压疾病患者胎盘组织中细胞外钙受体(CASR)mRNA及蛋白表达水平变化及其意义。方法采用免疫组化方法和免疫荧光及RT-PCR技术分别检测妊娠期高血压疾病患者65例(妊娠期高血压组22例,子痫前期轻度组24例,重度组19例)及健康足月孕妇30例(对照组)胎盘组织中CASR的定位、mRNA及蛋白表达水平。结果(1)CASR主要位于胎盘细胞滋养细胞、绒毛滋养细胞及血管内皮细胞上。(2)妊娠期高血压组患者胎盘组织中CASR的mRNA表达水平与对照组比较,差异有统计学意义(q=7.07,P=0.0004)。子痫前期轻度组和子痫前期重度组,两组比较,差异也有统计学意义(q=20.96,P<0.0001)。(3)CASR的蛋白在妊娠期高血压组患者胎盘组织中表达水平为59.0532±8.039,对照组为54.8585±4.3035,两组比较,差异无显著性(q=0.08,P=0.7759)。而子痫前期轻度组、子痫前期重度组两组比较,差异有统计学意义(q=16.18,P<0.0001)。结论妊娠期高血压疾病患者胎盘组织中CASR的mRNA表达激活及CASR的蛋白异常表达,导致妊娠期高血压疾病的发病和胎盘病理改变的发生发展。 Objective： To investigate the expression and significance of Calcium - Sensing Receptor on the placenta of Hyperten- sive Disorder Complicating Pregnancy. Methods ： Immunohistochemistry and Immunofluorescence and RT - PCR were used to investi- gate the expression of CASR proteins and mRNA in placenta from 22 cases of gestational hypertension, 24 cases of mild preeclampsia, 19 cases of severe preeclampsia and 30 cases of normal term pregnant women, Results： （1） Expression of CASR mRNA in placenta of gestational hypertension was significantly higher than that in normal term pregnancy, q =7. 07, P=0. 0004. Expression of CASR mRNA in placenta of mild preeclampsiawas significantly lower than that severe preeclampsia, q =20.96, P 〈0. 0001. （27 There was no significantly difference in CASR proteins level between gestational hypertension and normal term pregnancy （ q= 0. 08, P = 0. 7759）. Expression of CASR proteins in placenta of severe preeclampsia was significantly higher than that mild preeclampsia q = 16. 18, P 〈0. 0001. Conclusion： It suggested that the activation of CASR in the placental with hypertensive disorder complicating pregnancy and abnormal expressions of CASR in placenta at maternal - fetal （nterface are related to the pathogenesis of hypertensive disorder complicating pregnancy and typical pathological change in placenta.
作者 张延丽 江朵 任军
机构地区 山西医科大学第一医院
出处 《中国优生与遗传杂志》 2008年第9期37-39,共3页 Chinese Journal of Birth Health & Heredity
关键词 妊娠期高血压疾病 细胞外钙受体 胎盘 Hypertensive disorder complicating pregnancy Calcium -sensing Receptor （CASR） Placenta
分类号 R714.246 [医药卫生—妇产科学]
  • 相关文献

参考文献16

  • 1Arthur H Weston, Mais Absi, et al. Evidence in Favor of a Calcium - Sensing Receptor in Arterial Endothelial Ceils Studies With Calindol and Calhex 231 [ M]. Integrative Physiology, American Heart Association, Inc. 2005.
  • 2Christopher S Kovacs, Chrystal L, et al. Regulation of Murine Fetal - placental Calcium Metabolism by the Calcium - sensing Receptor[ J ]. J Clin Invest, 1998,101 (12) :2812 -2820.
  • 3Christopher S Kovacs. Calcium and bone metabolism in pregnancy and Lactation[J]. The Journal of Clinical Endocrinology & Metabolism,2001,86(6) :2344 -2348.
  • 4Brown E M, M Pollak, CE Seidman, JG Seidman, YH Chou, D Riccardi, SC Hebert. Calcium - ion - sensing cell - surface receptors [ J ]. N Engl J Med, 1995,333 : 234 - 240.
  • 5Chattopadhyay N, A Mithal, and EM Brown. The calcium -sensing receptor: a window into the physiology and path physiology of mineral ion metabolism[ J]. Endocrine Rev, 1996,17 : 289 - 307.
  • 6Brown EM, G Gamba, D Riccardi, M Lombardi, R Butters, O Kifor, A Sun, MA Hediger, J Lytton, and SC Hebert . Cloning and characterization of an extra cellular Ca^2 + - sensing receptor from bo- vine parathyroid [ J]. Nature, 1993,366 : 575 - 580.
  • 7De Wolf F, Robertson WB. The ultrastracture of acute atherosis in hypertensive pregnancy [ J]. American Journal of Clinical Pathology, 2001,123,164 - 74.
  • 8Kuang D, Yao Y, Lam J, Tsushima RG, Hampson DR. Cloning and characterization of a family C orphan G - protein coupled receptor [J]. J Neurochem, 2005 Apt, 93(2) :383 -91.
  • 9Shanklin DR, Sibai BM. Ultrastructural aspects of preeclampia. I. Placental bed and uterine boundary vessels[ J ]. American Journal of Clinical Pathology ,2001,161,735 - 41.
  • 10王笑云,王宁宁.钙受体激动剂在继发性甲状旁腺功能亢进治疗中的研究进展[J].中国药物与临床,2004,4(1):11-13. 被引量：2

二级参考文献6

共引文献6

同被引文献26

  • 1涂文,乔福元,许建平.TGF-β_1及TGFβRⅡ mRNA在子痫前期患者胎盘组织中的表达及意义[J].中国优生与遗传杂志,2006,14(11):14-15. 被引量：3
  • 2Ishihara N, Matsuo H, Murakoshi H, et al. Increased apoptosis in the syncytiotrophoblast in human term placenetas complicated by either preeclampasia or intrauterine growth retardation [ J ]. Am J Obstet Gynecol,2002,186( 1 ) : 158-166.
  • 3Reister F,Frank HG, Kingdom JC, et al. Macropbage-induced apoptosis limits endovascular trophoblast invasion in the uterine wall of preeclamptic women [ J ]. Lab Invest, 2001,81 ( 8 ) : 1143-1152.
  • 4Madazli R, Benian A, Ilvan S, et al. Placental apoptosis and adhesion molecules expression in the placenta and the maternal placental bed of pregnancies complicated by fetal growth restriction with and without pre-eclampsia[ J ]. J Obstet Gynaecol,2006,26 ( 1 ) : 5- 10.
  • 5Li P,Nijhawan D,Budihardjo I,et al. Cytochrome C and dATP-dependent formation Apaf-1/Caspase-9 complex initiates an apoptotic protease cascade[ J]. Cell, 1997,91 (4) :479-489.
  • 6Brockbank EC, Bridges J, Marshall C J, et al. Integrin beta 1 is required for the invasive behaviour but not proliferation of squamous cell carcinoma cells in vivo [ J ]. Br J Cancer, 2005,92 ( 1 ) : 102-112.
  • 7Wang J, Mayernik L,Armant DR. Trophoblast adhesion of the periimplantation mouse blastocyst is regulated by integrin signaling that targets phospholipase C [ J ]. Development Biology,2007,302 ( 1 ) : 143-153.
  • 8Lavee M, Goldman S, Daniel-Spiegel E, et al. Matrix metalloproteinase-2 is elevated in midtrimester amniotic fluid prior to the development of preeclampsia [ J ]. Reprod Biol Endocrinol, 2009,23 (7) :85.
  • 9Tayebjee MH, Karalis I, Nadar SK, et al. Circulating Matrix metalloproteinases-9 and tissue inhibitors of metalloproteinases-1 and-2 levels in gestational hypertension [ J]. Am J Hyper, 2005,18 (3) : 325 -329.
  • 10Wang S,Chen J, Beall M, et al. Expression of aquapofin 9 in human chorioamniotic membranes and placenta[ J]. Am J Obstet Gynecol,2004,191 (6) :2160-2167.

引证文献1

二级引证文献5

中国优生与遗传杂志
2008年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部