摘要
肝细胞性肝癌（ＨＣＣ）与ＨＢＶ整合密切相关，为研究ＨＢＶ－ＤＮＡ各片段在ＨＣＣ发生中的作用，用ＨＢＶ全长基因探针通过Ｓｏｕｔｈｅｒｎ转膜杂交分析了１７例ＨＣＣ标本中ＨＢＶ－ＤＮＡ的存在状态，并用ＨＢＶ片段基因探针和Ｎ－ｒａｓ、ｐ５３探针检测了其相应ｍＲＮＡ的转录情况。结果发现，５２．９％ＨＣＣ标本为纯整合型，４７．１％的标本为混合型。１７例ＨＣＣ中，Ｐｒｅｓ、ＨＢｓ、ＨＢｘ、ＨＢｃ转录率分别为４７．１％、５８．８％、７０．５％和４１．２％。９例纯整合型ＨＣＣ中，上述基因转录率分别为１１．１％、４４．４％、５５．６％和１１．１％，其中ＨＢｘ较ＨＢｃ、Ｐｒｅｓ差异有显著性（Ｐ＜０．０５），ＨＣＣ标本Ｎ－ｒａｓ、ｐ５３总转录率分别为８２．３％、６４．７％。提示ＨＢＶ－ＤＮＡ在ＨＣＣ细胞内主要以整合于染色体上的方式存在，而且整合片段可作为转录的模板，其蛋白产物可能通过影响Ｎ－ｒａｓ、ｐ５３在细胞调控中的功能促进肿瘤的形成。
Hepatocellular carcinoma(HCC) has high correlationship with hepatitis B virus (HBV) integration.In order to study the function of HBV subgenes in hepatocarcinogenesis,the HBV existence status was analysied by Southern blot hybridization using HBV 3.2 kb DNA probe in 17 HCC cases and mRNA was detected with HBV subgenes and Nras,p53 probes in specimens extracts. The results showed that the integrated type of HCC was 52.9%,both integrated and free type was 47.1%;the transcription percentage of 17 HCC cases of subgene pres,HBs,HBx,HBc and Nras,p53 were 47.1%,58.8%,70.5%,41.2%,82.3% and 64.7% respectively;in 9 integrated type cases,the transcription rate of subgene pres,HBs,HBx,HBc were 11.1%,44.4%,55.6%,11.1% respectively and HBx had obvious difference compared with pres and HBc(P<0.05).HBVDNA mainly integrated into chromosome DNA in HCC cells and integrated fragments may be transcripted;the protein of HBV subgenes could interact with p53,Nras and influence the latter function in cell growth and regulation,hence promote the hepatocarcinogenesis.
出处
《山东医科大学学报》
1997年第4期284-289,共6页
Acta Academiae Medicinae Shandong
基金
山东省科委资助项目
