摘要
目的观察17β-雌二醇（E2）、辛伐他汀和异搏定一硝酸甘油复合溶液（V-G）在短时间内对体外培养的人脐带静脉内皮细胞（HUVECs）合成一氧化氮（NO）功能的影响。方法HUVECs分别用17β-E2（10^-5mol／L）、辛伐他汀（10^-9mol／L）和V-G溶液孵育30、60、120min后应用试剂盒测定NO的生成量，内皮细胞的总一氧化氮合酶（tNOS）活性，并用RT-PCR方法测定对内皮细胞一氧化氮合酶（eNOS）mRNA表达的影响。结果17β-E2、辛伐他汀和V-G溶液作用30、60和120min后可以使NO的生成量增加（P〈0．05）；17β-E2和辛伐他汀在作用30、60min时能够增加tNOS活性，但作用时120min tNOS的活性降低（P〈0．05）；V—G溶液在作用30、60和120min时均能够增加HUVECs的tNOS活性（P〈0．05）；17β-E2、辛伐他汀和V-G溶液在作用120min后对eNOS的mRNA水平无影响（P〉0．05）。结论短时间内17β-E2、辛伐他汀和V—G溶液能够影响内皮细胞的tNOS，增加内皮细胞的NO合成功能，V—G溶液的保护效果要优于前两者。
Objective To investigate the effect of 17β-estrogen（E2）, simvastatin and verapamil-nitroglycerin （V-G） solution on the synthesise of NO in human umbilical vein cells （HUVECs）. Methods HUVECs were treated with 17β-E2, simvastatin and V-G solution for 30 min, 60 min and 120 min, respectively. NO production and tNOS activity were measured with NO and tNOS reagent box. Levels of mRNA of endothelial nitric oxide synthase（eNOS） were examined with reverse transcription polymerase chain reaction （RT-PCR） after the HUVECs were incubated for 120 min. Results There was a significant increase of NO production after treating HUVECs with 17β-E2, simvastatin and V-G solution for 30, 60 and 120 minutes. The level of tNOS activity was significantly increased after treated with 17β-E2 and simvastatin for 30 and 60 minutes, but it declined after 120 minutes. V-G solution could increase tNOS activity at 3 time points.There were no difference in eNOS mRNA levels among 17β-E2, simvastatin and V-G solution at 120 min （ P 〉 0.05）. Conclusion NO production of HUVECs could be increased by 17β-E2, simvastatin and V-G solution.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2008年第4期255-257,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
一氧化氮
雌二醇
心血管药物
硝酸甘油
药代动力学
Nitric oxide Estradiol Cardiovascular agents Nitroglycerin Pharmacokineties
