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新型环糊精手性固定相对氟西汀对映体的拆分 被引量：2

Separation of Fluoxetine Enantiomers on New β-Cyclodextrin Derivative Bonded Chiral Stationary Phase
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摘要 用分步法制备全苯异氰酸酯基-β-环糊精键合硅胶手性固定相.在高效液相色谱仪上,对氟西汀对映体进行拆分,并探讨流动相组成及配比、离子强度、pH值、流速和柱温等影响分离的因素.结果表明,在自制手性固定相上,外消旋氟西汀得以拆分;最优的流动相为甲醇-0.2%醋酸三乙胺缓冲液(体积比35∶65,pH 4.0),当流速为1.0 mL/min和柱温为35℃时可达到基线分离. The perphenylcarbamoylated-β-cyclodextrin bonded silica gel chiral stationary phase （CSP） is prepared step by step. Then, fluoxetine enantiomers are separated by HPLC. The effects of mobile phase composition, ionic strength, pH, flow rate and column temperature on separation are studied systematically. By this CSP, fluoxetine enantiomers can be separated. The optimal mobile phase composition is methanol --0.2% TEAA （pH 4.0）, and baseline resolution can be achieved under flow rate of 1.0 mL/min and at 35 ℃.
作者 周婕 施秀芳 张振中
机构地区 郑州大学药学院
出处 《郑州大学学报（理学版）》 CAS 2008年第3期121-124,共4页 Journal of Zhengzhou University:Natural Science Edition
基金 教育部高等学校博士学科点专项科研基金资助项目 编号20040335045
关键词 环糊精衍生物 手性固定相 氟西汀对映体 拆分 高效液相色谱 β-eyelodextrin derivative chiral stationary phase fluoxetine enantiomer separation HPLC
分类号 O652 [理学—分析化学]
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参考文献5

  • 1Cramer F. Chromatographic resolution of racemates on chiral stationary phases: 1. Influence of the supramolecular structure of cellulose triacetate[J]. Angew Chem, 1952, 64: 136-148.
  • 2Takeichi T, Toriyama H, Shimura S. Determination of fluoxetine and norfluoxetine in human plasma by HPLC with ultraviolet detection in psychiatric patients[J]. J High Resol Chromatogr, 1995, 18:179-186.
  • 3Zhang L F, Chen L, Lee T C, et al. A facile route into 6A-mono-ω-alkenylcarbamido-6A-deoxy-perfunctionalised cyclodextrin., key intermediate for further reactive functionalisations [J]. Tetrahedron: Asymmetry, 1999, 10 (21): 4107-4113.
  • 4Lai H X, Ng S C. Preparation and chiral recognition of a novel chiral stationary phase for high-performance liquid chromatography, based on mono(6A-N-allylamino-6A-deoxy)-perfunctionalized β-cyclodextrin and covalently bonded silica gel [J]. J Chromatogr A, 2004, 1031: 135-142.
  • 5Yee L, Wong S, Skrinska V. Chiral high-performance liquid chromatography analysis of fluoxetine and norfluoxetine in rabbit plasma, urine and vitreous humor using an acetylated beta-cyclodextrin column[J]. Journal of Analytical Toxicology, 2000, 24: 651-655.

同被引文献16

  • 1李永红,于梅艳,刘宏民.高效液相色谱法测定β-氨基醇类药物中间体的含量及ee值[J].郑州大学学报（医学版）,2009,44(2):427-429. 被引量：6
  • 2Sharif NA, Xu SX. Binding affinities of ocular hypotensive beta-blockers levobetaxolol, levobunolol, and timolol at endogenous guinea pig beta-adrenoceptors [ J ]. J Ocul Pharmcol Ther, 2004,20 ( 2 ) : 93.
  • 3Aoyama Y, Motoki M, Hashimoto M. Effect of various anti-glaucoma eyedrops on human corneal epithelial cells [ J ]. Nippon Ganka Gakkai Zasshi ,2004,108 ( 2 ) :75.
  • 4中华人民共和国药典委员会.中华人民共和国药典2005年版[M].北京:化学工业出版社,2005:附录176.
  • 5Chen Xiaoping,Zhou Zhiming,Yuan Hao,et al.Preparation and chiral recognition of a mono[6A-N-1-(2-hydroxy)-phenylethylimino-6A-deoxy]-beta-cyclodextrin HPLC stationary phase[J].J Chromatogr Sci,2008,46(9):777-782.
  • 6Li Xia,Zhou Zhiming,Li Dai,et al.Synthesis and enantioseparation characteristics of a novel mono-6-deoxy-(2,4-dihydroxybenzimide)-β-cyclodextrin as a chiral stationary phase in high-performance liquid chromatography[J].Talanta,2011,86:452-456.
  • 7Pihlainen K,Kostiainen R.Effect of the eluent on enantiomer separation of controlled drugs by liquid chromatography-ultraviolet absorbance detectionelectrospray ionization tandem mass spectrometry using vancomycin and native-β-cyclodextrin chiral stationary phases[J].Journal of Chromatogr A,2004,1033(1):91-99.
  • 8Carbonnier B,Janus L,Morcellet M.High-performance liquid chromatographic enantioseparation of flavanones on 2-hydroxy-3-methacryloyloxypropyl beta-cyclodextrin copolymer coated silica phase[J].Journal of Chromatogra Sci,2005,43(7):358-361.
  • 9Poon Y F,Muderawan I W,Ng S C.Synthesis and application of mono-2A-azido-2A-deoxyperphenylcarbamoylatedβ-cyclodextrin and mono-2A-azido-2A-deoxyperacetylatedβ-cyclodextrin as chiral stationary phases for high-performance liquid chromatography[J].Journal of Chromatogr A,2008,1101(1/2):185-197.
  • 10Chen Lei,Zhang Lifeng,Ching C H,et al.Synthesis and chromatographic properties of a novel chiralstationary phase derived fromheptakis(6-azido-6-deoxy-2,3-di-ophenylcarbamoy-lated)-β-cyclodextrin immobilized onto aminofunctionalized silica gel via multiple urea linkages[J].Journal of Chromatogr A,2002,950(1/2):65-74.

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郑州大学学报（理学版）
2008年 第3期

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