摘要
目的通过两种不同移植途径研究骨髓间质干细胞治疗急性心肌梗死可行性及疗效对比。方法骨髓间质干细胞培养传至5代后，制备成浓度4×10^6/ml悬液备用。39只SD大鼠均结扎冠状动脉左前降支，制备成急性心肌梗死模型，随机分为3组每组各13只。Ⅰ为对照组：从尾静脉注射生理盐水。Ⅱ为直接经心外膜移植组：模型制备成功后1-3 h梗死周边区分六点注射骨髓间质干细胞，每点50μl。Ⅲ为通过静脉移植组：模型制备成功后24h，从尾静脉注射骨髓间质干细胞0.5 ml（含2×10^6个）连续7 d，Ⅰ和Ⅱ组注射等量生理盐水7 d作为对照。5周后，观察3组的大鼠死亡率以及心脏的结构和功能。结果Ⅰ组有3只大鼠死亡，其他组无死亡。与Ⅰ组相比，Ⅱ组和Ⅲ组的左心室结构与功能明显改善（P〈0.05），Ⅱ组和Ⅲ组相比无明显差别（P〉0.05）。结论骨髓间质干细胞移植治疗急性心肌梗死有效；静脉注射与直接经心外膜心肌注射两种途径无明显差别，静脉注射移植相对简单易行。
Objective To study the feasibility and curative effects of derived bone marrow mesenchymal stem cells （MSC）transplantation in treating acute myocardial infarction （AMI）by different transplanting ways. Methods MSC suspension was prepared in 4 × 10^6/ml concentration after derived bone marrow MSC were cultured till 5^th generation. Thirty-nine Sprague-Dawley （SD）rats with their left anterior descending coronary artery ligature were divided randomly into three groups. Group Ⅰ as control group while normal saline was injected via rat tail vein; group Ⅱ as epicardium transplantation group while 50 μl MSC was transplanted directly via microinjection around 6 infracted areas after AMI models were prepared for 1 -3 hours ;group Ⅲ as vein transplantation group while 50 μl MSC were injected for 7 days after 24 h of AMI. Equal amount of normal saline were injected for 7 days in group Ⅰ and group Ⅱ as control. Fatality and cardiac construction and function of rats in 3 groups were investigated after 5 weeks. Results Three rats died in group Ⅰ. Compared to group Ⅰ ,left heart function had been obviously improved in group Ⅱ and Ⅲ （P 〈 0.05）. There was no difference between Ⅱ and Ⅲ （ P 〉 0.05 ）. Conclusion MSC transplantation into AMI rats can improve heart function obviously. There is no great difference between transplanting MSC via rat tail vein and via microinjection around infracted area directly. Transplanting MSC via rat tail vein is simple and effective.
出处
《同济大学学报（医学版）》
CAS
2008年第4期13-16,共4页
Journal of Tongji University(Medical Science)
基金
上海市自然科学基金资助项目(04ZR141)
关键词
骨髓间质干细胞
急性心肌梗死
细胞培养
大鼠
mesenchymal stem cells
acute myocardial infarction
cell culture
rats
