摘要
目的观察完全弗氏佐剂(CFA)外周刺激后,大鼠中脑导水管周围灰质(PAG)中胶质纤维酸性蛋白(GFAP)、小胶质细胞标记物(Iba)以及细胞因子IL-β、TNF-α的表达变化。方法结合行为学检测,反转录-聚合酶链式反应(RT-PCR)、Western blotting以及免疫组织化学的方法,观察了大鼠后爪注射CFA后疼痛感觉的变化,并检测了PAG中上述标记物和细胞因子在基因水平和蛋白水平的表达变化。结果注射CFA后,注射侧后爪出现热痛过敏和机械性触诱发痛;大鼠的热痛过敏14d基本恢复正常,但机械性触诱发痛持续到21d时仍未恢复正常;星形胶质细胞标记物GFAP在急性期和慢性期有显著增加;小胶质细胞的标记物CD14、细胞因子(IL-β、TNF-α)在急性期、亚急性期和慢性期均有增加。结论小胶质细胞的激活可能与炎性疼痛的起始相关,而星形胶质细胞可能与疼痛的维持相关,细胞因子表达的增加可能对痛觉过敏的发生起重要作用。
Objective To observe the activation of astrocytes, microglia and expression of proinflammatory cytokines in the periaqueductal gray （PAG） by Complete Freunds Adjuvant （CFA）-induced inflammatory pain. Methods Heat hyperalgesia and mechanical allodynia were observed and the expressions of mRNA, protein of glial markers, and proinflammatory cytokines in the PAG were examined following intraplantar administration CFA in right hindpaw of the rat by behavioral testing, reverse transcription polymerase chain reaction （RT-PCR）, Western blotting, and immunohistochemistry. Results CFA injection induced chronic thermal hyperalgesia and mechanical allodynia. After injection, the thermal hyperalgesia was recovered on Day 14, but the mechanical allodynia lasted more than 21 days. Microglial marker （Cluster of differentiation 14, CD14） and proinflammatory cytokines （lnterleukinin-1β, tumor necrosis factor-α） showed a significant increase in their expression during all phases （acute, subacute and chronic） of inflammation. GFAP was observed only in PAG at subacute and chronic phases of inflammation. Conclusion The activation of microglia and astrocytes may be involved in the initiation and maintenance of inflammatory pain. Enhanced cytokines expression might play a role in eliciting behavioral hypersensitivity.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第4期460-465,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30500153)
江苏省高校自然科学研究计划资助项目(05KJB180100)
