摘要
目的探讨肿瘤细胞侵袭转移能力的改变对肿瘤细胞化疗敏感性的影响。方法Westernblotting法比较筛选出的人胃癌细胞MKN-28S(亚系)与MKN-28(母系)中E-Cadherin、MMP-2蛋白表达;transwell体外侵袭实验比较两系侵袭潜能。采用MTT法比较两系对阿霉素(ADM)、丝裂霉素(MMC)、5-氟尿嘧啶(5-Fu)、紫杉醇(PTX)4种化疗药物的敏感性。结果筛选出亚系的E-Cadherin蛋白表达与母系相比显著减弱、而MMP-2蛋白表达则显著增强;显微镜下细胞计数示亚系侵袭至基质胶膜背面的数量较母系明显增多(P<0.05);MTT示两系对5-Fu、ADM、MMC的敏感性差异有显著性(P<0.05),而两系对PTX的敏感性差异无显著性(P>0.05)。结论肿瘤细胞侵袭转移能力的改变可以影响肿瘤细胞对化疗药物的敏感性,同一遗传背景下的高侵袭转移亚系较母系耐药性更强。
Objective To explore effects of alteration in tumor cells＇ invasiveness and metastasis on its chemotherapeutic sensitivity. Methods Protein expression level of E -Cadherin and MMP-2 in MKN-28S and MKN-28 cell lines was assessed by Westem blot. The invasiveness was assessed by penetration through a matrigel-coated filter. MTr assays were used to detect sensitivity of two cell lines by ADM, MMC, 5-Fu, and PTX treatment. Result Protein expression level of E-cadherin was significantly decreased and that of MMP-2 significantly increased in MKN-2 8S10 cell line compared with MKN-28 cell line. The mount of sub-cells penetrating through matrigel-coated filter was significantly increased compared with parent cells under light microscope （P〈0.05）. The sensitivities in both cell lines were significantly different for ADM, MMC and 5-Fu （P〈0.05）, but not for FTX（P〉0.05）. Conclusions Alteration in tumor cells＇ invasiveness and metastasis had an effect on its chemotherapeutic sensitivity to certain agents. Subcell line with identical genetic backgrounds of high invasiveness and metastasis had stronger drug resistance, compared with the parent one.
出处
《南华大学学报（医学版）》
2008年第3期313-315,共3页
Journal of Nanhua University(Medical Edition)
关键词
胃癌
侵袭转移
化疗敏感性
Gastric carcinoma
Invasion and metastasis
Chemotherapy sensitivity
