摘要
以稻瘟病菌粗毒素提取液为选择压,选取籼稻材料缙2B、缙恢36和缗恢10进行抗稻瘟病突变体的筛选.结果表明:1)在成熟胚诱导产生愈伤组织过程中,2,4-D是诱导愈伤的关键因子,3种材料在附加2mg/L 2,4-D的NB培养基(NBD_2)上诱导产生的愈伤较好.2)粗毒素对3种材料的胚根、胚芽、愈伤组织的生长都有着明显的抑制作用,表明粗毒素具有一定的致病力,其毒性并不与浓度完全成正比关系,而是在一定范围内随浓度的增加而增加.在毒素浓度为45%时,缗2B胚根和胚芽生长受到抑制的程度最大,而缙恢36和缙恢10则在30%浓度中生长受到明显抑制.3)粗毒素对3种材料的愈伤组织成活率及再生频率有着明显的抑制作用,其程度随浓度的增加而增大,当稻瘟病粗毒素浓度为60%时,愈伤组织不生长,并变褐死亡,最终选择45%的毒素浓度进行2次继代抗性筛选并获得再生植株.
Under the selection pressure of crude toxin extracted from Pyricularia oryzae, three indica rice （Oryza sativa L. suhsp, indica）, Jin2B, Jinhui36 and Jinhuil0, which possess eminent characters and are sensitive to rice blast, were used to induce callus for screening the blast-resistant somatic mutant. The results indicated that： 1） 2, 4-D was the key factor of inducing callus for mature embryo, and callus represented good quantities and qualities in the medium of NB with 2 mg/L 2,4-D. 2） The crude toxin inhibited evidently growth of radicel and embryo, subculture and differentiation, so the crude toxin was capability of diseases-producing, the toxicity was not corresponding to the toxin concentration, hut the inhibiting enhanced with the increasing of the toxin in some range. The growth of radicel and embryo of Jin2B was inhibited most fiercely under 45% toxin concentration, while Jinhui36 and Jinhuil0 in 30%. 3） Frequency of living callus and regeneration was restrained evidently in three materials, with the increasing of the toxin concentration, the inhibiting got enhanced. When the medium was added 60% crude toxin, calli couldn＇t develop and become brown, even dead. Finally 45% toxin concentration was chosen for subculture and screening, and plantlet regeneration was obtained.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期52-56,共5页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市水稻玉米重大专项资助项目
关键词
籼稻
稻瘟病菌
粗毒素
细胞突变体筛选
rice （Oryza sativa L. subsp, indica）
Pyricularia oryzae
crude toxin
screening somatic mutant
