摘要
目的:探讨先兆子痫、正常妊娠女性辅助性T细胞(Th)亚群功能变化和外周血Th1、Th2细胞百分率与外周血单个核细胞(PBMC)培养上清液中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)浓度的相关性。方法:应用流式细胞仪检测外周血Th1、Th2细胞百分率,ELISA法测定PBMC体外培养上清液中细胞因子IFN-γ和IL-4浓度。结果:与正常非妊娠妇女相比,先兆子痫患者Th1细胞百分率、Th1/Th2比值明显升高,差异有显著性(P<0.05或P<0.01);正常妊娠女性Th1细胞百分率、Th1/Th2比值明显下降,差异有非常显著性(P<0.01)。与正常妊娠女性相比,先兆子痫患者Th1细胞百分率、Th1/Th2比值明显升高,差异有显著性(P<0.01)。外周血Th1细胞百分率与PBMC培养上清液中IFN-γ浓度、Th2细胞百分率与IL-4浓度、Th1/Th2与IFN-γ/IL-4的比值均呈显著性正相关。结论:先兆子痫患者Th1细胞亚群功能增强,Th1/Th2细胞亚群功能失衡,导致病理妊娠。正常妊娠女性Th1细胞亚群功能明显减弱,对妊娠具有保护作用。流式细胞仪检测外周血Th1、Th2细胞百分率与ELISA法测定PBMC体外培养上清液中细胞因子IFN-γ和IL-4浓度具有良好的相关性。
Objective To investigate the functional changes of Th cell subpopulation in peripheral blood from normal pregnant and preeclamptic women and correlation between the percentage of Thl and IFN-γ concentration and that between the percentage of Th2 and IL-4 concentration. Methods The percentage of Thl cells and that of Th2 in peripheral blood were measured using flow cytometry, and the concentrations of IL-4 and IFN-γ in the supernatant from which peripheral blood mononuclear cells were cultured in vitro were determined by enzyme-linked immunosorbent assay. Results The percentage of Thl cells and the Th1/Th2 cell ratio were significantly higher in preeclamptic patients than in normal nonpregnant or pregnant while were markedly lower in normal pregnant women than in normal nonpregnant women. The percentage of Thl cells was positively related with IFN-γ concentration in culture supernatant, so were the percentage of Th2 cells with IL-4 concentration and the Th1/Th2 ratio with the IFN-γ concentration/IL-4 concentration ratio. Conclusions Enhancement of Thl cells and Th1/Th2 imbalance leads to pathological pregnancy in preeclamptic patients. The markedly decreased Thl cells are beneficial for normal pregnancy. The percentage of Thl is associated with IFN-γ concentration, so is that of Th2 with IL-4 concentration.
出处
《实用医学杂志》
CAS
2008年第18期3120-3122,共3页
The Journal of Practical Medicine
基金
浙江省温州市科技局科研项目(编号:Y2003A060)
