摘要
目的探讨子宫内膜异位症(EMS)患者异位及在位子宫内膜基质细胞在白细胞介素-1β(IL-1β)诱导培养条件下RANTES mRNA和蛋白表达模式的变化情况。方法对2006年9月至2007年6月南京医科大学第一附属医院收治的11例EMS患者的异位、在位内膜基质细胞和同期5例正常内膜基质细胞行分离培养,分别加入5、10、20μg/L的IL-1β刺激培养异位内膜基质细胞,选择最佳浓度的IL-1β刺激培养异位、在位和正常内膜基质细胞,采用RT-PCR及ELISA法从转录及转录后水平观察趋化因子RANTES表达模式的变化情况。结果10μg/LIL-1β诱导异位内膜基质细胞分泌RANTES蛋白能力强于5μg/L组,稳定性优于20μg/L组。异位内膜基质细胞于IL-1β刺激12h开始RANTES mRNA表达较在位及正常内膜显著增强,在位内膜基质细胞自48h开始RANTES mRNA表达较正常内膜显著增强。异位内膜自刺激培养24h开始RANTES蛋白分泌量明显高于在位内膜和正常内膜,96h可高达(926.52±97.19)ng/L,在位内膜自刺激培养60h开始RANTES蛋白分泌量较正常内膜显著增加,正常内膜基质细胞RANTES分泌量维持于(39.19±14.29)^(56.88±17.28)ng/L。结论EMS患者的异位、在位子宫内膜与正常子宫内膜相比,其RANTES表达特性已经发生了改变;异位内膜基质细胞可能是EMS患者腹腔液中高浓度RANTES的来源之一。
Objective To explore the difference of RANTES expression in stromal cells of ectopic, autologous eutopic and normal endometrium induced by IL-1β. Methods The stromal cell of ectopic implants was exposed to various concentrations （5,10,20ug/L）of IL-1β and the 10ug/L of IL-1β was cocultured with ectopic implants, eutopic and normal endometrium. RANTES mRNA in the cells was detected with RT-PCR and concentration of RANTES protein in the culture su- pernatant was measured with a sandwish ELISA. Results The 10 ug,/L of IL-1β elicited the highest secretion of RANTES in ectopic implants. The RANTES expressed by the stromal cell of ectopic implants was increased significantly compared with autologous eutopic and normal endometrium. The RANTES secreted by the stromal cell of autologous eutopic endometrium was increased significantly compared with normal endometrium. Conclusion Our findings confirm that the different expressions of RANTES mRNA and protein exist among ectopic, autologous eutopic and normal endometrium. The ectopic endometrial stromal cells may contribute to the high secretion of RANTES in the pelvic fluid of women with endometriosis.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2008年第8期586-589,共4页
Chinese Journal of Practical Gynecology and Obstetrics
基金
国家自然科学基金(30600671)
江苏省自然科学基金(BK2006577)
