摘要
宇佐美曲霉木聚糖酶(XynⅡ)是G/11族高比活木聚糖酶的一种,通过S179C定点突变,发现酶最适反应温度由原来的50℃提高到52℃;50℃热处理,半衰期由原酶的23 min延长到突变后酶的70 min,热稳定性提高到原来的3倍;最适反应pH无变化。
Xylanase from Aspergillususamii belongs to family-11 and is a type of xylanase that has high relative activity. Utilizing the method of S179C-directed mutagenesis, we improved its optimum temperature from 50℃ to 52℃. It maintained 50% residual activity after 70 min of incubation at 50℃, while the residual activity of the native xylanase was 50% only after 23 min at the same incubation temperature. The themostability was enhanced by 3-fold. Optimum pH value, however, did not present notable changes
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第7期55-58,共4页
Food and Fermentation Industries
关键词
木聚糖
木聚糖酶
热稳定性
定点突变
xylan, xylanase, thermostability, site-directed mutagenesis
